Features & Benefits

• 2x concentrated reagents minimize pipetting steps, simplify reaction assembly and improve accuracy

• Superior assay sensitivity and specificity with ultrapure AccuStart enzyme technology – maximum-yielding Taq DNA polymerase mutant controlled by stringent, multi-epitope antibody hot start

• Supports efficient vortex mixing with proprietary anti-foaming formulation

• SuperMix version provides maximum dye concentration for robust optical signal with small amplicons (i.e. microRNAtemplated cDNA)

PerfeCTa SYBR Green SuperMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

PerfeCTa SYBR^® Green SuperMix is a user-friendly, 2X concentrated reaction mix that simplifies setup and is pre-blended with reference dye for optimized product performance. This proprietary buffer technology stabilizes a high concentration of SYBR Green I dye to ensure maximum optical signal with low abundance or small targets (such as microRNA). Successful detection with a non-specific, dsDNA intercalating dye requires precise target amplification as off-target primer elongation will contribute to overall fluorescent signal and lead to over-reported relative abundance values. This reagent is powered by a highly-processive, ultra-pure Taq DNA polymerase mutant with stringent, ultra-pure AccuStart™II antibody hot start technology that allows ambient room-temperature setup and maximal enzyme kinetics after rapid, irreversible denaturation at 95°C.

Contents

Single-tube, 2X concentrated reagent containing:

• Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, and dTTP.

• AccuStart II Taq DNA Polymerase

• SYBR Green I dye

• Proprietary enzyme stabilizers and performance-enhancing additives.

• Titrated reference dye (if applicable).

Storage & Handling

Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
After thawing, mix thoroughly before using.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

Catalog #: 95056-500 -- 500 x 50 μL rxns (10 x 1.25 mL)

Catalog #: 95056-02K -- 2000 x 50 μL rxns (1 x 50 mL)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Manual PerfeCTa SYBR Green SuperMix, Low ROX