Features & Benefits

• Superior Sensitivity – higher yields optimized for lower viral RNA inputs

• Tough Tested – tolerant to a wide range of PCR inhibitors

• Multiplexing – Supports highly sensitive detection for up to four targets

• Broad Dynamic Range – Reliable data from your precious samples every time

qScript 1-Step Virus ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

qScript 1-Step Virus ToughMix is a 2x, ready-to-use, master mix for rapid detection of RNA viruses such as Flu-A, Flu-B, and SARS-CoV-2, using one-step, or single-tube reverse transcription quantitative PCR (RT-qPCR). It has been optimized for maximum sensitivity to enable reliable quantification of very low input quantities of RNA using dual-labeled hydrolysis probe detection chemistries such as TaqMan® probes in single or multiplexed assay formats. qScript 1-Step Virus ToughMix contains all required components for RT-qPCR except RNA template and primer/probe assays.

qScript 1-Step Virus ToughMix is powered by an engineered reverse transcriptase with reduced RNase H activity and improved activity and stability at higher temperatures, that includes a RT Hot-Start technology to suppresses non-specific primer extension by the RT before cDNA synthesis. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis. These features combined with a stringent mAb hot-start Taq polymerase and ToughMix reagent technology to neutralize many common PCR inhibitors, provide reproducible low-copy quantification as well as extended room temperature stability of fully assembled reactions for automated reaction assembly.

qScript 1-Step Virus ToughMix does not contain an internal reference dye.

Contents

• 2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride,

• qScript XLT reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase,

• AccuVue blue qPCR dye, and stabilizers

Storage & Handling

• Store components in a constant temperature freezer at -25°C to -15°C.

• Repeated freezing and thawing do not affect RT-qPCR performance.

• For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

Catalog #: 95131-100 -- 95131-100 x 20 μL rxns (1 x 1 mL)

Catalog #: 95131-500 -- 95131-500 x 20 μL rxns (5 x 1 mL)

Catalog #: 95131-02K -- 95131-2000 x 20 μL rxns (1 x 20 mL)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Manual qScript 1-Step Virus ToughMix