Features & Benefits

• Tough Tested – Overcomes common inhibitors including polysaccharides, heme/hemoglobin, humic acid, melanin

• Flexible – Use fast or standard qPCR cycling conditions

• Broad dynamic range- Reliable data from your precious samples every time

• Multiplexing enabled – Supports highly sensitive detection for up to four targets

qScript XLT 1-Step RT-qPCR ToughMix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

qScript XLT One-Step RT-qPCR ToughMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5'-hydrolysis probes. First-strand cDNA synthesis and subsequent PCR amplification are carried out seamlessly in the same reaction mixture with optimized 1-step thermal cycling parameters.

This kit is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in minimal reaction volumes and accelerated thermal cycling rates. The only necessary user-supplied materials for RT-qPCR is RNA sample and probe assay. It is compatible with all types of molecular probe assays including dual-labeling strategies.

Elevating cDNA synthesis reaction temperature to 50-55°C during one-step RT-qPCR improves primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.

Contents

2X concentrated One-Step Master Mix containing:

• Reaction buffer with optimized concentrations of molecular-grade MgCl2, dATP, dCTP, dGTP, dTTP

• qScript XLT reverse transcriptase

• RNase inhibitor protein

• AccuStart II Taq DNA Polymerase

• Inert AccuVue™ plate loading dye

• Proprietary enzyme stabilizers and performance-enhancing additives

• Titrated reference dye (if applicable)

Storage & Handling

Store components in a constant temperature freezer at -25°C to -15°C protected from light upon receipt.
Repeated freezing and thawing does not affect RT-qPCR performance.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

Catalog #: 95132-100 -- 100 x 20 µL rxns (1 x 500 µL)

Catalog #: 95132-500 -- 500 x 20 µL rxns (5 x 500 µL)

Catalog #: 95132-02K -- 2000 x 20 μL rxns (1 x 20 mL)

Catalog #: 95132-05K -- 5000 x 20 µL rxns (1 x 50 mL)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Manual qScript XLT One-Step RT-qPCR ToughMix