Features & Benefits

• HiFi DNA polymerase engineered to minimize amplification bias

• Increased amplification efficiency resulting in higher yields

• Uniform coverage across challenging AT- and GC-rich regions

• Robust amplification from input DNA as low as 250 pg

• Cost-effective alternative to KAPA HiFi with improved performance

sparQ HiFi PCR Master Mix is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

sparQ HiFi PCR Master Mix is a high-fidelity, high-efficiency PCR master mix for NGS workflows requiring DNA library amplification prior to sequencing. The included primer mix allows amplification of DNA libraries flanked by adapters containing the P5 and P7 sequence elements required for Illumina^® sequencing platforms. The hot-start, proofreading DNA polymerase used in the sparQ HiFi PCR Mater Mix is specifically engineered to improve library amplification efficiency while reducing PCR-derived artifacts, resulting in higher library yields and better coverage uniformity. This kit supports low DNA input from 250 pg and efficient amplification of AT- and GC rich regions with minimal bias.

Contents

• 2X HiFi PCR Master Mix

• Primer Mix

• HiFi Enhancer

Storage & Handling

Store components in a constant temperature freezer at -25°C to -15°C upon receipt.
Refer to the product label for applicable expiration date.

Catalog #: 95192-050 -- 50 rxn

Catalog #: 95192-250 -- 250 rxn

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Manual sparQ HiFi PCR Master Mix