Features & Benefits

• Fast, easy single-tube solution completes library prep in 2.5 hours

• Suitable for a wide range of input amounts from as low as 250 pg

• Optimized chemistry ensuring superior library prep sensitivity and efficiency

• Higher library yields compared to other library prep kits

• High efficiency enables PCR-free workflow from 100 ng input

sparQ DNA Library Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

sparQ DNA Library Prep Kit is optimized for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms. The simplified protocol speeds up library prep to 2.5 hours with minimal hands-on time and accommodates DNA input amounts from 250 pg to 1 ug. DNA polishing reactions are streamlined into a single step to convert fragmented DNA into 5'-phosphorylated and 3'-dA-tailed DNA fragments. This is followed by high efficiency adapter ligation in the same tube. PCR-free workflows are enabled from 100 ng of starting material. If library amplification is required, the HiFi PCR Master Mix and Primer Mix ensure even amplification with minimal bias.

Storage & Handling

Store kit components in a constant temperature freezer at -25°C to -15°C upon receipt.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

Catalog #: 95191-024 -- 24 rxn

Catalog #: 95191-096 -- 96 rxn

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Manual sparQ DNA Library Prep Kit