Features & Benefits

• High quality libraries in 2.5 hours from 1 ng – 1 μg of input DNA

• Tunable and reproducible fragmentation size range

• Simple, convenient 2-step workflow with minimal hands-on time

• Novel chemistry and high-fidelity amplification minimizing bias

• Superior sequence coverage uniformity and low duplication rate

sparQ DNA Frag & Library Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.

Description

The sparQ DNA Frag & Library Prep Kit is optimized for enzymatic fragmentation of DNA and streamlined construction of high quality libraries for sequencing on Illumina NGS® platforms. The simple, convenient 2-step workflow can be completed in 2.5 hours with minimal hands-on time and accommodates DNA input amounts from 1 ng to 1000 ng. The DNA fragmentation and polishing reactions are combined in a single step to generate 5'-phosphorylated and 3'-dA-tailed fragments. This is followed by high efficiency adapter ligation in the same tube. PCR-Free workflows are enabled from 100 ng of starting material. If library amplification is required, the HiFi PCR Master Mix and Primer Mix ensure even amplification with minimal bias.

Storage & Handling

Store kit components in a constant temperature freezer at -25°C to -15°C upon receipt.
For lot specific expiry date, refer to package label, Certificate of Analysis or Product Specification Form.

Catalog #: 95194-024 -- 24 rxns

Catalog #: 95194-096 -- 96 rxns

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Manual sparQ DNA Frag & Library Prep Kit