Product Overview

Lonza's Cryopreserved HUVECs are shipped as frozen primaries. They are guaranteed to express CD31/105 and survive in culture through 15* population doublings (*except HUVEC-XL™ guaranteed through 5 PD).

All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

HUVEC-XL size consists of 10 million cells/amp achieved by pooling donors. This option is designed to support studies that require large volume of cells while still offering cost-benefits to researchers.

Our pooled vs. single donor options allow researchers to study or exclude donor variability from their experiments. Depending upon the study, pooled version is typically utilized to reduce donor variability while single donor option allows researchers to assess donor-specific differences.

Not All HUVECs Are Created Equal – Which Type Is Suitable for Your Research?

• CC-2517 (single donor), CC-2519 (pooled donor) - Both isolated in EGM™ Media, in absence of defined growth factors. Contains BBE. No exogenous VEGF

• CC-2935 (single donor) - Isolated in EGM^™ - Plus Media: Improved version of part number CC-2517. Offers faster proliferation rates than HUVEC isolated in EGM™ Media. No exogenous VEGF

• C2517A (single donor), C2519A (pooled donor) 00191027 (XL size) - Isolated in EGM™-2 Media, in presence of growth factors such as VEGF, rhFGF, rhEGF, and others. Does not contain BBE

• C2517AS (single donor), C2519AS (pooled donor) - Pre-screened HUVECs in EGM™-2 Media. Screened for angiogenesis markers and test positive for expression of eNOS, Axl, Tie-2 and VEGFr2

Benefits

• All cells test negative for mycoplasma, bacteria, yeast, and fungi

• HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots

• Certificate of Analysis provided for every cell lot

Content

1 x Cryopreserved ampule HUVEC containing ≥ 10 million cells/vial

Recommended Medium

Lonza EGM™-2 Endothelial Cell Growth Medium-2 BulletKit™

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Technical Information and Instructions for Proper Use of Endothelial Cells

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved