Product Overview

Human Cardiac-Related Fibroblasts include Aortic Adventitial Fibroblasts (AoAF) isolated from the tunica external of the ascending or descending aorta and cardiac fibroblasts (NHCF-A and -V) isolated from the atrium or ventricle. Cells are isolated from normal, adult heart tissue. Cryopreserved cells are shipped at passage 2. 

Clonetics™ NHCF-V & -A are guaranteed through 5 population doublings and express ≥90% collagen I and ≤10% Von Willibrand factor VIII after thaw. Clonetics™ AoAF are guaranteed through 10 population doublings and are negative for alpha smooth muscle actin. All cells test negative for mycoplasma, bacteria, yeast and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis (CoA) is provided for each cell lot purchased.  

Benefits

• Assured for experimental use up to 15 population doublings 

• Express ≥90% collagen I and ≤10% Von Willibrand factor VIII

• All cells test negative for mycoplasma, bacteria, yeast, and fungi

• HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots

• Certificate of Analysis provided for every cell lot

Applications

• Vascular diseases

• Neointimal formation

• Vascular remodeling

• Atherosclerosis

• Hypertension

• Myocardial repair

• Extracellular maintenance during cardiac cell / tissue growth

• Cardiac Fibrosis Paracrine signalling

• Toxicology

• Gene expression

Content

1 x Cryopreserved ampule NHCF-V containing ≥ 500,000 cells/vial

Recommended Medium

Lonza FGM™-3 Cardiac Fibroblast Growth Medium-3 BulletKit™

Catalog #: CC-2904

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Technical Instructions for culturing Clonetics™ Human Fibroblasts Cells

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved