Product Overview

Human bronchial smooth muscle cells are isolated from the major bronchia of asthmatic donors.                                                   

Asthma is an inflammatory disorder of the airways, which causes attacks of wheezing, shortness of breath, chest tightness, and coughing in patients. Degrees of severity are unique to individual patients as are responses to drug treatments. Adhesion of eosinophils to bronchial epithelial cells, and the interaction of airway smooth muscle and sub-epithelial fibroblasts play a critical role in asthma pathology. During an asthma attack bronchiole smooth muscle tissue can constrict and decrease the flow of air in the airways. The amount of air flow can further be decreased by inflammation or excess mucus secretion. 

Cryopreserved Diseased Bronchial Smooth Muscle cells stain positive for alpha smooth muscle actin and negative for von Willebrandfactor VIII. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitisC are not detected for all donors and/or cell lots. A Certificate of Analysisis provided for each cell lot purchased.

Benefits

• Gene expression pathways

• Phenotypic changes to compounds

• Transfect diseased cells

• Co-culture with immune cells

• Airway remodeling studies

• Cellular interactions

Content

1 x Cryopreserved Ampule with ≥500,000 cells/vial

Recommended Medium

Lonza SmGM™- 2 Smooth Muscle Cell Growth Medium -2 BulletKit™

Catalog #: 194850

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Instructions for culturing Clonetics™ Human Smooth Muscle Cells

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved