Lonza Rat cardiac myocytes
Cryopreserved ampule of Rat Neonatal Cardiomyocytes containing ≥4 million cells. Catalog #: R-CM-561
Product Overview
Neonatal, ventricular Rat Cardiomyocytes (P1-3) are high quality primary myocyte cells prepared by standardized methods, and are ready for immediate culture upon thaw.
One vial of cardiomyocyte cells contains ≥ 4 million viable cells at ≥ 85% purity. This will seed into 10 or more wells of a 24-well plate and 26 or more wells of a 96-well plate using the recommended plating densities and medium.When thawed and cultured you will obtain high viability, with excellent morphology and connectivity, and cells will display beating at 24 hours in culture.
Each lot of these cells test positive for functional syncytium formation, and stain positive for actinin. Cells also test negative for mycoplasma and bacteria. Primary cardiac myocyte cells need an appropriate substrate to adhere and survive. The preferred substrate is nitrocellulose.
Recommended medium: RCGM™ BulletKit™ (CC-4515)*
*RCGM™ Rat Cardiomyocyte Growth Medium is specifically formulated for the growth and survival of rat neonatal cardiomyocytes in culture. Lonza offers customers a fully validated and optimized cardiomyocyte medium and growth factors to complement our Clonetics™ Rat Cardiomyocytes, R-CM-561, as a complete system to study cardiac biology/toxicology.
Benefits
Ready-To-Use
No tissue dissection or animal handling required
Increased data reproducibility
Characteristic morphology and staining tests confirm tissue origin and cell type
Negative for mycoplasma, bacteria, yeast and fungi
Certificate of Analysis (CoA) is available for every batch
Applications
Cardiovascular diseases
Hypertension
Inflammation
Cell contraction
Cardiac cell physiology
Ion channel flux
Cardiac lipid metabolism and lipotoxicity
Cardiac ischemia and injury
Cardiac apoptosis
Cardiac cytotoxicity
Tissue engineering
Content
≥ 4 million cryopreserved cells per ampule
Recommended Medium
Catalog #: R-CM-561
Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.