Product Overview

Clonetics™ Rat Cerebellar Granular Cells are isolated from cerebellar cortex of adult Sprague Dawley rats at postnatal day 8. Cerebellar granular cells utilize glutamate as their neurotransmitter and as such, are the only excitatory neurons within the cerebellar cortex.

All other neuronal cell types employ GABA as a neurotransmitter. Granular cells arise during the latter stages of neuronal development of the cerebellar cortex and are the most abundant single neuronal type in the mammalian brain. These are highly homogeneous populations of granular cells containing less than 5% GABAergic neurons, as assessed by GABA immunoreactivity.

Recommended Media: PNGM™-A BulletKit™ (CC-4512)

Benefits

• Ready-To-Use 

• >95% pure granular cells, with glia

• No tissue dissection or animal handling required 

• Increased data reproducibility

• Characteristic morphology and staining tests confirm tissue origin and cell type

• Negative for mycoplasma, bacteria, yeast and fungi

• Certificate of Analysis (CoA) is available for every batch 

Applications

• Electrophysiology

• Neurotransmitter release

• Ion channels

• Glutamate receptor biology

• Neuronal apoptosis and toxicity

• Nitric Oxide and cGMP pathway

• Neurite outgrowth

Content

≥ 4,000,000 cryopreserved cells per ampule

Recommended Medium

Lonza PNBM™ Primary Neuron Basal Medium, 200 mL

Lonza PNGM™ Primary Neuron Growth Medium SingleQuots™ Supplements and Growth Factors

Catalog #: R-Cb-503

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Instructions for proper use and culture of Rat Brain Cerebellar Neurons

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved