Lonza Irradiated mouse embryonic fibroblasts
Cryopreserved ampule of Irradiated Mouse Embryonic Fibroblasts containing ≥2 million cells. Catalog #: M-IFB-482
Product Overview
Irradiated Mouse primary embryonic fibroblasts (iMEF) are dissociated from day 14 and 15 CD1 mouse embryos, expanded and then inactivated using gamma radiation.
Each vial contains at least 2 million cells. iMEF are convenient and easy to use “feeder cells” commonly co-cultured with embryonic and pluripotent stem cell cultures.
Recommended Media: Lonza's DMEM High Glucose w/ L-Gln, 500 mL (Part 12-604F) containing 10% FBS (Sigma-Aldrich Part F6178)
Benefits
Ready-To-Use
No tissue dissection or animal handling required
Increased data reproducibility
Characteristic morphology and staining tests confirm tissue origin and cell type
Negative for mycoplasma, bacteria, yeast and fungi
Certificate of Analysis (CoA) is available for every batch
Applications
Serve as “feeder cell” monolayer in embryonic (ES) and induced pluripotent (iPSC) stem cell cultures
Secrete important growth factors into culture medium to support ES and iPSC undifferentiated state
Act as cellular matrix to support ES cell growth
Content
≥ 2 million cryopreserved, gamma irradiated cells per ampule
Catalog #: M-IFB-482
Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.