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Cytek® Aurora

A prodigy that's taking flow cytometry to the next level of performance and flexibility # full spectrum technology

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High Level Overview

The Cytek Aurora leverages full spectrum technology to provide unprecedented flexibility to scientists, enabling the use of a wide array of new fluorochrome combinations without reconfiguring the system for each application. The Aurora system delivers high-resolution data at the single-cell level to resolve the most challenging cell populations, such as cells with high autofluorescence or low levels of expression of key biomarkers, regardless of assay complexity. Learn More About Full Spectrum Cytometry
 

SpectroFlo® software offers an intuitive workflow from QC to data analysis with technology-enabling tools that simplify running applications.

 

So Many Colors

Exceptional Sensitivity

A New Level of Flexibility

Up to 40 colors demonstrated including fluorochromes with emission spectra in close proximity to each other. Sensitivity redefined using state of the art optics and low noise electronics. No changing optical filters for any fluorochrome. Use any commercially available fluorochrome excited by the onboard lasers.

 

Technology

With up to five lasers, three scattering channels, and 64 fluorescence channels, the Aurora system is highly flexible, intuitive and ultra-sensitive. With its intuitive optical design, compact footprint and upgradeability from 3 to 5 laser configurations, the Aurora system suits every laboratory’s needs from simple to high complexity applications.

 

 

 

 

 

 

 

 

Proprietary high sensitivity Coarse Wavelength Division Multiplexing (CWDM) semiconductor detector arrays enables more efficient spectrum capture for dyes emitting in the 365-829 nm range. CWDM full spectrum technology unlocks each fluorochrome to express themselves fully across the entire light spectrum.

 

 

 

 

The state-of-the-art optics and low-noise electronics provide excellent sensitivity and resolution. Flat-top beam profiles, combined with a uniquely designed fluidics system, translate to outstanding performance at high sample flow rates.

 

 

 

 

 

 

 

 

 

Performance Data

Autofluorescence Extraction

Aurora Full Spectrum Flow Cytometer Autofluorescence Extraction

The Aurora system’s implementation of full spectrum flow cytometry enables the use of autofluorescence extraction to further improve data clarity. Certain sample types, such as yeast and tumor samples, present the challenge of high autofluorescence. For these challenging applications involving highly autofluorescent particles, let the software's autofluorescence extraction tool bring new levels of resolution.

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Small Particle Detection

 

Cytek Aurora Full Spectrum Flow Cytometer Bead Performance DataWith its onboard 100 mW 405 nm laser and highly sensitive violet SSC detector, Aurora opens the door to a wide variety of small particle applications, taking what was once hidden and placing it in full view.

 

 

 

 

Deep Immunoprofiling

The Cytek Aurora’s use of full spectrum flow cytometry combined with the SpectroFlo® software’s real-time unmixing capability provides greater fluorochrome choice and panel flexibility and allows users to quickly visualize data and statistics. All this results in the ultimate flow cytometry solution for deep immunoprofiling, from 24 colors all the way to 40 colors.

 

24 Colors

The three-laser configuration provides outstanding multi-parametric data for a wide array of applications. Check out how markers and fluorochromes in a 24-color panel designed for identification of circulating cell subsets in human peripheral blood look when run on the Aurora system.

Violet Excited Dyes Emission Spectra Blue Excited Dyes Emission Spectra Red Excited Dyes Emission Spectra
Violet Excited Dyes Emission Spectra Blue Excited Dyes Emission Spectra Red Excited Dyes Emission Spectra

 

 

40 Colors

Until recently, developing fluorescence-based flow cytometry assays with 40 colors has been merely aspirational, with many turning to competing technologies for high-parameter applications. No longer. With 64 fluorescence detectors and only 5 lasers, the Cytek Aurora now has the capability to resolve up to 40 colors in combination. Cytek has developed a 40-color human immunophenotyping panel acquired from a single tube, with outstanding resolution.

 

 

For more information, please review the CYTEK AURORA PRODUCT BROCHURE or contact us (cs@medikonia.com) directly to discuss your specific application.