Product Overview

BEGM™ Bronchial Epithelial Cell Growth Medium is specially designed to support the growth of human primary bronchial epithelial cells. BEGM™ BulletKit™ is serum-free and has been optimized to grow cells from normal and diseased tissues.

Published research shows BEGM™ BulletKit™ has expanded applications. Our customers have used BEGM™ BulletKit™ to culture well-established cell lines such as BEAS-2B, home-grown bronchial epithelial cells and supporting co-culture models with breast cancer cell lines such as MCF-7 and MDA-MB-231. Refer to the published literature in the 'Data' tab.

BEGM™ is an optimized and readily available media kit providing researchers the confidence and the convenience of a system that works to support their cells. This is especially important for researchers who are utilizing cell lines as a stepping stone to primary cell culture. Working with the same BEGM™ Media between cell lines and primary NHBE can reduce variability within the experiments and simplify the shift for researchers. In addition, if primary cells are utilized as a side-by-side control with normal cell lines or home-grown bronchial epithelial cells, the BEGM™BulletKit™ Media may also offer cost-benefit since customers can use the same media to support multiple cell types.

Here are publications out of many where our customers have used BEGM™ Growth Media with normal bronchial epithelial cells:

• El Najjar, et al. (2015). PLoSONE 10(2) demonstrates culturing of BEAS-2B bronchial epithelial cell line in BEGM™ Media to examine the effects of cathepsin and furin proteolytic enzymes on viral fusion protein activation in cells

• Hagiwara, et al. (2015). Nature demonstrates BEGM™ Media can ideally support co-culture of Lonza's HUVEC and NHBE in a 3D environment.

• Rider, et al. (2015). PLoS ONE 10(1) used BEGM™ Media to culture primary human bronchial epithelial cells obtained from non-transplanted normal lungs

Purchase our Bronchial Epithelial Cell Culture Kit (catalog#. CC-2540B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of bronchial epithelial cells (catalog #CC-2540) and BEGM™ BulletKit™ Growth Media (catalog#. CC-3170).

Content

1 x BEBM™ Basal Medium (CC-3171), 500 mL

1 x BEGM™ SingleQuots™ Supplement Pack (CC-4175) containing:
1 x Orange Cap Vial with BPE, 2.00 mL
1 x Lilac Cap Vial with Insulin, 0.50 mL
1 x Natural Cap Vial with Hydrocortisone, 0.50 mL
1 x Red Cap Vial with GA-1000, 0.50 mL
1 x Amber Vial with Retinoic Acid, 0.50 mL
1 x Natural Cap Vial with Transferrin, 0.50 mL
1 x Amber Vial with Triiodothyronine, 0.50 mL
1 x Amber Vial with Epinephrine, 0.50 mL
1 x Green Cap Vial with hEGF, 0.50 mL

Catalog #: CC-3170

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Instructions for Proper Use and Culture of Airway Epithelial Cells

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved