Product Overview

Stem cells possess the unique ability to differentiate into multiple lineages of cells including osteoblasts. Lonza’s hMSC Osteogenic Differentiation BulletKit™ Medium is a serum containing medium designed to induce osteogenic differentiation of human bone marrow derived mesenchymal stem cells (hMSC) into mature, functionally active osteoblasts in as few as 14 days. Differentiation can then be validated by a variety of methods including the use of Lonza’s OsteoImage™ Bone Mineralization Reagent which provides a quantitative value related to the amount of osteogenic differentiation.

Partial List of Cells Differentiated with hMSC Osteogenic Differentiation BulletKit™ Medium*:

• Human bone marrow derived stem cells

• Human umbilical cord blood derived stem cells

• Human umbilical cord matrix derived stem cells

• C57BL/6 mice bone marrow derived stem cells

• Human adipose derived stem cells

• Human periodontal ligament derived stem cells

• Human hamstring tendon derived stem cells

The hMSC Osteogenic Differentiation BulletKit™ Medium provides a differentiation medium guaranteed to induce osteogenic differentiation of bone marrow derived hMSCs into mature, functionally active osteoblasts; Each bottle of the hMSC Osteogenic Differentiation BulletKit™ Medium contains enough differentiation medium to fully differentiate up to 750,000 bone marrow derived hMSCs [approximately 25 wells in a 6-well plate format] into mature, functionally active osteoblasts.

The hMSC Osteogenic Differentiation Medium is offered as a BulletKit™ Medium (catalog no. PT-3002) which includes both the basal media and the necessary supplements for osteogenic differentiation of bone marrow derived hMSCs.

Lonza guarantees the performance of cells only if appropriate media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. 

*List of cells generated from customer data. Lonza only routinely uses this medium with Lonza’s human bone marrow derived mesenchymal stem cells and can therefore only guarantee the successful use of this media with these cells.

Content

1 x hMSC Osteogenic Differentiation Basal Medium (PT-3924), 170 mL

1 x hMSC Osteogenic Differentiation SingleQuots™ Supplements Kit (PT-4120)

Catalog #: PT-3002

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Instructions - Human Mesenchymal Stem Cell Systems

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved