Product Overview

The X-VIVO™ 10 Media formulations are designed to support the generation of LAK cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor peripheral blood lymphocytes (PBL) at 1.0 – 3.0 × 10⁶ cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peri pheral blood lymphocytes are incubated for 3 – 10 days at a density of 1.0 – 6.0 × 10⁶ cells/ml in the presence of 100 – 1,000 Cetus units of rIL-2.

All BioWhittaker™ Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact us for more details.

Benefits

• Superior media for most hematopoietic cells types including dendritic cells, lymphocytes, monocytes, macrophages and granulocytes.

• Formulations only contain human derived components

• More regulatory friendly as human component is replaced by a recombinant one

• Highly cited in scientific literature

• Clinical proven formulations providing process safety and constancy

• High productivity resulting in a high viable cell yield

• Serum Free media result in a more defined process and result in limited immune response

• Efficient transgene expression Desirable T-cell phenotype

Applications

• Proliferation of peripheral blood lymphocytes

• Proliferation of tumor infiltrating lymphocytes

• Cryopreservation of human tissue

• Cultivation of human monocytes and macrophages

• Cultivation of stem cells

• Cultivation of dendritic cells

Content

1L bottle

Catalog #: BE04-380Q

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

References - X-Vivo™

BioWhittaker™ Specialty Media – Maximize Your Cell Growth and Productivity

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved