For Whole Blood Collection and Preservation of Cell-Free Circulating DNA and RNA

Norgen's cf-DNA/cf-RNA Preservative Tubes are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping.

• - Preservation and isolation of both cf-DNA and cf-RNA from a single tube

• - Fixative-free preservative, no cross-linking of DNA

• - Preserve cf-DNA/ct-DNA for 30 days at ambient temperature and for up to 8 days at 37°C

• - Preserve cf-RNA for 30 days at ambient temperature

• - Preserve Circulating Tumour Cells (CTCs) for 14 days at ambient temperature

• - No plasma volume loss after shipping/transportation

• - Prevent hemolysis allowing better separation of plasma

• - Prevent apoptosis of blood cells and fragmentation of genomic DNA

• - Produce high quality/quantity of plasma cf-DNA/ct-DNA/cf-RNA

• - Vacuumed to draw 8.4 mL of blood in 10 mL tubes

• - Instructional video available: Proper mixing instructions - View on YouTube

• - The procedure for plasma isolation using this kit is compliant with ISO 20186-3:2019

For research use only and NOT intended for in vitro diagnostics.

cf-DNA/cf-RNA Preservative Tubes

Norgen's cf-DNA/cf-RNA Preservative Tubes are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen's Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free cf-DNA/ct-DNA/cf-RNA in a very small elution volume. Plasma recovered from Norgen's cf-DNA/cf-RNA Preservative Tubes is also compatible with any other cf-DNA and/or cf-RNA purification method. The purified cf-DNA, ct-DNA and cf-RNA are compatible with any downstream application assays, including PCR, qPCR, rt-qPCR, methylation-sensitive PCR, Southern Blot analysis, gene expression analysis, microarrays and NGS.

This product is for research use only and not for use in diagnostic procedures.

Performance

Norgen's cf-DNA/cf-RNA Preservative Tubes abridge the collection/processing of whole blood for the subsequent purification of cf-DNA and/or cf-RNA. The cf-DNA/cf-RNA Preservative Tubes preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA/RNA into plasma. Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma. The cf/ct DNA and cf-RNA levels are stable for up to 30 days at room temperature. The cf-DNA is also stable for up to 8 days at 37°C. When blood collected on Norgen's cf-DNA/cf-RNA tubes is processed for plasma recovery no buffy coat will be generated and the Circulating tumor cells (CTCs) will be located at the bottom of the tube with the blood sediment. CTCs are stable for 14 days at room temperature where it can be extracted from the blood sediment or from whole preserved blood for cell sorting or for cf/DNA or cf-RNA purification.

Plasma samples produced from the whole blood sample collected into Norgen’s cf-DNA/cf-RNA Preservative Tubes and purified using Norgen's Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen's Plasma/Serum RNA Purification Kits yield concentrated, high-quality and inhibitor-free cf-DNA/ct-DNA or cf-RNA for any downstream application.

Kit Specifications

Tube Size:

• 16 x 100mm

Tube Type:

• PET Tube

Blood Draw Volume:

• 8.4 mL in 10 mL Tubes

Preservative Volume:

• 1.6 mL

Recovered Plasma Volume:

• Approximately 6-7 mL (not affected by shipping)

Anticoagulant:

• Proprietary

Preservative:

• Non formaldehyde - Proprietary preservation agent

Length of cf-DNA Preservation:

• Up to 30 days at room temperature (15-25°C)

• Up to 8 days at 37°C

Length of cf-RNA Preservation:

• Up to 30 days at room temperature (15-25°C)

Length of CTCs Preservation: 

• Up to 14 days at room temperature (15-25°C)

Catalog #: 63950

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

cf-DNA/cf-RNA Preservative Tubes - Protocol (50 Tubes)

Supplementary Protocols

 cf-DNA/cf-RNA Preservative Tubes - Mixing Instructions

Isolation of White Blood Cells (WBCs) / Circulating Tumour Cells (CTCs) from cf-DNA/cf-RNA Preservative Tubes

RNA/DNA/Protein Purification from Leukocytes recovered from cf-DNA / cf-RNA Preservative Tubes

Genomic DNA Purification from Leukocytes recovered from cf-DNA/cf-RNA Preservative Tubes

Total RNA Purification from Leukocytes recovered from cf-DNA/cf-RNA Preservative Tubes

Product Information Sheets

Proper Mixing Procedure

Application Notes

Assessing the Effect of Simulated Shipping on Cell-Free DNA in Blood Collected in Norgen, Streck and EDTA Tubes

Safety Data Sheets

cf-DNA/cf-RNA Preservative Tubes - Safety Data Sheet

Posters

Proper Mixing Procedure

KRAS-dependent and independent mechanisms of progressive disease [...] monitoring on circulating cell free DNA (cfDNA)

Product Flyers

cf-DNA/cf-RNA Preservative Tubes