Number of Targets Detected: 

 60

 Species Detected:

 Human

 Compatible Sample Types:

  - Cell Culture Supernatants

  - Cell Lysates

  - Other Body Fluids

  - Plasma

  - Serum

  - Tissue Lysates

 Design Principle:

Sandwich-based

 Method of Detection:

Fluorescence Laser Scanner

 Quantitative/Semi-Quantitative:

Quantitative

 Solid Support:

Glass Slide

 Size:

1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Protein Information:

Gene Symbols:

Product Features:

  - More cost-effective than traditional ELISA

  - High specificity and system reproducibility

  - Suitable for diverse sample types

  - Low sample volume requirement: 100 µL or less

  - Get results same day (6-hour processing time)

  - Well-suited for high throughput assays

  - Q Analyzer software provides one-step computation

Species Detected:

Human

 Suggested Application:

Multiplexed Protein Detection;  Biomarker Screening;  Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

 Kit Components:

 - Human Angiogenesis Array Q1000 Slide(s)

  - Blocking Buffer

  - Wash Buffer 1

  - Wash Buffer 2

  - Lyophilized Standard Mix

  - Biotinylated Detection Antibody Cocktail

  - Streptavidin-Conjugated Fluor

  - Slide Washer/Dryer

  - Adhesive Plastic Strips

  - Manual

 Other Materials Required:

  - Distilled or deionized water

  - Small plastic boxes or containers

  - Pipettors, pipette tips and other common lab consumables

  - Orbital shaker or oscillating rocker

  - Aluminum foil

  - Gene microarray scanner or similar laser fluorescence scanner

 Protocol Outline:

  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6 .Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8 .Scan with a gene microarray laser scanner
  9 .Perform densitometry and analysis