Product Overview

The SG Cell Line 384-well Nucleofector™ Kit is one of our three kits suited for high-throughput transfection of cell lines when working with the 384-well HT Nucleofector™ System.

This kit has been determined to be optimal for transfecting following cell types:

• Ba/F3

• Colo-205

• H4

• NIH/3T3

• Raji, Ramos

• THP-1

• WEHI

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 2 x 384 or 10 x 384 reactions.

Benefits

• High transfection efficiencies in difficult-to-transfect cell lines

• Cells maintain functionality post transfection

• Seamless integration into automated liquid handling environments

• Conditions are transferable between 4D-Nucleofector™ System, 96-well Shuttle™ System and 384-well Nucleofector™ System

Applications

• Transfection of low cell numbers down to 2 x 10⁴ cell lines

• High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries

Content

2x384 reactions:

22.5 mL SG Cell Line Nucleofector™ Solution
5 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 384-well Nucleocuvette™ Plate (20 μl)

10x384 reactions:

90 mL SG Cell Line Nucleofector™ Solution
20 mL Supplement 1
50 µg pmaxGFP™ Vector (1 μg/μl in 10 mM Tris pH 8.0)
10 x 384-well Nucleocuvette™ Plate (20 μl)