For purification of total RNA (including microRNA) from all types of tissues including fiber-rich tissues

• Extract high quality & quantity total RNA including miRNA 

• Isolate from a wide variety of animal tissues 

• No phenol step required - isolate all RNA in one fraction

• Compatible with tissues stored in RNAlater® or Trizol®  

• Convenient & fast spin column format

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Animal Tissue RNA Purification Kit

This kit is suitable for the isolation of total RNA from a range of animal tissues such as liver and spleen, as well as difficult fibrous tissues such as heart, muscle, intestine, etc.  Briefly, the tissue of interest is first lysed using Buffer RL, followed by treatment with the provided Proteinase K which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins, and connective tissues. The purified RNA is of the highest quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS, and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Kit Specifications

Storage Conditions and Product Stability
The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date shipment.

Catalog #: 25700

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Animal Tissue RNA Purification Kit - Protocol (50 prep)

Safety Data Sheets

Animal Tissue RNA Purification Kit - Safety Data Sheet

Product Flyers

Total RNA Purification