For the rapid purification of microRNA without phenol

• Isolate and extract high quality & quantity miRNA in 30 minutes

• Two column kit- eliminate large RNA on the first column and capture microRNA on a second column

• Elute microRNA in 25 µL ready for miRNA profiling

• Process a wide range of samples- cell, tissue, bacteria, bodily fluids, etc.

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

microRNA Purification Kit

This kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from cultured animal cells, small tissue samples, bacterial cells, plants, and blood.

Two columns are provided with this kit. The first column captures large RNA, while the small RNA are captured on a second column and are eluted concentrated in 25 µL of nuclease-free water.

The small RNA can be used in various downstream applications relating to miRNA profiling, gene regulation, and functional analysis. The eluted RNA is ready for RT-qPCR, microarrays, and NGS applications.

Background

These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long and regulate gene expression by binding to mRNA molecules and affecting their stability or translation.

Kit Specifications

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

Catalog #: 21300

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

microRNA Purification Kit - Protocol (25 prep)

Application Notes

Non-Organic-Based Isolation of Mammalian microRNA using Norgen’s microRNA Purification Kit

Effect of RNA Isolation Method on microRNA Quantity and Quality in Plasma: A Comparative Study

Safety Data Sheets

microRNA Purification Kit - Safety Data Sheet

Posters

Silicon Carbide As A Novel RNA Affinity Medium With Improved Sensitivity and Size Diversity - microRNA 2008

Product Flyers

Total RNA Purification