Rapid purification of total RNA - including microRNA - from small input amounts

• Extract high quality & quantity total RNA including miRNA

• No phenol step required - isolate all RNA in one fraction

• Elute extracted total RNA in small volume (20 µL)

• Bind & elute all RNA irrespective of size or GC content, without bias.

• Efficiently extract small RNA irrespective of GC content

• Very sensitive & linear down to a few cells without the need for carrier RNA

• Convenient & fast spin column format

• Isolate from a wide variety of specimens

• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Total RNA Purification Micro Kit

This kit extracts total RNA from low cell number samples and elutes in a convenient 20 µL elution for a number of downstream applications.

This kit is suitable for the isolation of total RNA from a range of samples including small input of cells, needle biopsies, LCM, CTC and other low cell number samples. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Kit Specifications

* for fibrous tissue, an additional Proteinase K treatment is required

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Catalog #: 35300 -- 50 preps

Catalog #: 35350 -- 250 preps

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Total RNA Purification Micro Kit - Protocol (50 and 250 prep)

Supplementary Protocols

Total RNA Purification Micro Kit Supplementary Protocol For Dried Blood Spot RNA Isolation - Supplementary Protocol

Total RNA Purification Micro Kit - Supplementary Protocol for Exosomal RNA Purification from Exosomes Already Purified

Safety Data Sheets

Total RNA Purification Micro Kit - Safety Data Sheet