Anti-Pembrolizumab ELISA

This kit is a sandwich-based bridging ELISA for the quantitative measurement of the human antibody against Pembrolizumab in serum, plasma, and cell culture supernatants.

Specifications

Size: 1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Targets Detected: Pembrolizumab

Protein Name/ Synonyms: Pembrolizumab

Quantitative/ Semi-Quantitative: Quantitative

Compatible Sample Types: Cell Culture Supernatants, Plasma, Serum

Solid Support: 96-well Microplate

Method Of Detection: Colorimetric

Design Principle: Sandwich-based

Research Area: Antibody Drug Development, Anti-Drug Antibody (ADA) Detection

Sensitivity: 0.007 ng/ml

Detection Range: 0.074 ng/ml - 54 ng/ml

Recommended Dilution (Serum/ Plasma): 8 fold

Application Note

Kit Components

• - Pre-Coated 96-well Strip Microplate

• - Wash Buffer

• - Lyophilized Standard

• - HRP-Conjugated Pembrolizumab

• - TMB One-Step Substrate

• - Stop Solution

• - Assay Diluent

Other Materials Required

• - Microplate reader capable of measuring absorbance at 450nm

• - Precision pipettes to deliver 2 µl to 1 ml volumes

• - Adjustable 1-25 ml pipettes for reagent preparation

• - 100 ml and 1 liter graduated cylinders

• - Absorbent paper

• - Distilled or deionized water

• - Log-log graph paper or computer and software for ELISA data analysis

• - Horizontal orbital microplate shaker

• - Tubes to prepare standard and sample dilutions

Protocol Outline

• 1. Prepare all reagents, samples and standards as instructed in the manual.

• 2. Add 100 µl of standard sample to each well. Incubate 2 hours at room temperature.

• 3. Add 100 µl prepared HRP-conjugated Pembrolizumab to each well. Incubate 1 hour at room temperature,

• 4. Add 100 µl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.

• 5. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.

Storage/ Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Catalog #: EAD-AnPemb-1 -- 1 Plate Kit

Catalog #: EAD-AnPemb-2 -- 2 Plate Kit

Catalog #: EAD-AnPemb-5 -- 5 Plate Kit

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Manual

Safety Data Sheet