Denosumab ELISA

This kit is a snadwich-based bridging ELISA for the quantitative measurement of Denosumb in serum, plasma, and cell culture supernatants. 6 - 8 week lead time.

Specifications

Size: 1 Plate Kit, 2 Plate Kit, 5 Plate Kit

Targets Detected: Denosumab

Protein Name / Synonyms: Denosumab

Quantitative / Semi-Quantitative: Quantitative 

Compatible Sample Types: Cell Culture Supernatants, Plasma, Serum

Solid Support: 96-well Microplate

Method Of Detection: Colorimetric

Design Principle: Sandwich-based

Research Area: Antibody Drug Development, Drug Antibody (DA) Detection

Applications Notes

Kit Components

• - Pre-Coated 96-well Strip Microplate

• - Wash Buffer

• - Lyophilized Standard

• - Biotinylated Detection Antibody

• - Streptavidin-Conjugated HRP

• - Stop Solution

• - Assay Diluent

Other Materials Required

• - Microplate reader capable of measuring absorbance at 450 nm

• - Precision pipettes to deliver 2 µl to 1 ml volumes

• - Adjustable 1 - 25 ml pipettes for reagent preparation

• - 100 ml and 1 liter graduated cylinders

• - Absorbent paper

• - Distilled or deionized water

• - Log-log graph paper or computer and software for ELISA data analysis

• - Horizontal orbital microplate shaker

• - Tubes to prepare standard and sample dilutions

Protocol Outline

• 1. Prepare all reagents, samples and standards as instructed in the manual.

• 2. Add 100 µl of standard or sample to each well. Incubate 2 hours at room temperature.

• 3. Add 100 µl prepared biotinylated antibody to each well. Incubate 1 hour at room temperature.

• 4. Add 100 µl prepared Streptavidin solution. Incubate 45 minutes at room temperature.

• 5. Add 100 µl TMB One-Step Substrate Reagent to reach well. Incubate 30 minutes at room temperature.

• 6. Add 50 µl of Stop Soution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Catalog #: EAD-Deno-1 -- 1 Plate Kit

Catalog #: EAD-Deno-2 -- 2 Plate Kit

Catalog #: EAD-Deno-5 -- 5 Plate Kit
 

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Manual

Safety Data Sheet