Human IL-27 IQELISA™ Kit

RayBio® Human Immunoquantitative (PCR-Based) IL-27 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Specifications

Application Notes

Kit Components

• - IL-27 Microplate (Item A)**: 96 well PCR plate coated with anti-Human IL-27

• - Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution

• - Standards (Item C): 2 vials of recombinant Human IL-27

• - Assay Diluent B (Item E): 15 ml of 5x concentrated buffer

• - Detection Affinity Reagent for IL-27 (Item F): 2 vials of a 4x concentrated solution of anti-Human IL-27 affinity reagent

• - IQELISA„™ Detection Reagent (Item G): 1 ml of a 10x concentrated stock

• - Primer Solution (Item I): 1.5 ml vial

• - PCR Master Mix (Item J): 1.4 ml vial

• - PCR Preparation buffer (Item K): 1 ml vial of 10x concentrated buffer

• - Final Wash Buffer (Item L): 10 ml vial of 10x concentrated buffer

**The PCR plate used is a 0.2 ml, non-skirted 96-well plate (ThermoFisher, cat. no.: AB0600). Please ensure compatibility with your PCR machine prior to purchase. For additional information contact technical support (cs@medikonia.com).

Other Materials Required

• - Real-time PCR instrument, Bio-Rad recommended

• - Precision pipettes to deliver 2 µl to 1 ml volumes

• - Adjustable 1-25 ml pipettes for reagent preparation

• - 100 ml and 1 liter graduated cylinders

• - Absorbent paper

• - Distilled or deionized water

• - Log-log graph paper or computer and software for data analysis

• - Tubes to prepare standard or sample dilutions

• - Heating block or water bath capable of 80°C

Protocol Outline 

• 1. Prepare all reagents, samples and standards as instructed

• 2. Add 25 µl standard or sample to each well. Incubate for 1.5 - 2.5 hours at room temperature

• 3. Add 25 µl detection antibody to each well. Incubate 1 hour at room temperature

• 4. Add 25 µl of IQELISA„™ Detection Reagent to each well. Incubate 1 hour

• 5. Add 10 µl Primer solution and 10 µl of PCR master mix to each well

• 6. Run real-time PCR

Storage/Stability

May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Catalog #: IQH-IL27-1 -- 1 Plate Kit

Catalog #: IQH-IL27-2 -- 2 Plate Kit

Catalog #: IQH-IL27-5 -- 5 Plate Kit

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Manual

Safety Data Sheet