Human Activin R2A IQELISA™ Kit
RayBio® Human Immunoquantitative (PCR-Based) Activin R2A ELISA Kit for cell culture supernatants, plasma, and serum samples.
Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
---|---|
Species | Human |
Quantitative/Semi-Quantitative | Quantitative |
Protein Name / Synonyms | Activin receptor type-2A (EC 2.7.11.30) (Activin receptor type IIA) (ACTR-IIA) (ACTRIIA) |
Accession Number | P27037 |
Gene Symbols | ACVR2A|ACVR2 |
Gene Id | 92 |
Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
Solid Support | 96-well Microplate **The PCR plate used is a 0.2mL, non-skirted 96-well plate (ThermoFisher, cat. no.: AB0600) |
Method Of Detection | qPCR |
Design Principle | Sandwich-based |
Sensitivity | 0.12 ng/ml |
Detection Range | 0.12 ng/ml - 500 ng/ml |
Recommended Dilution (Serum/Plasma) | 3 fold |
The RayBio® Immuno Quantitative Enzyme Linked ImumunoSorbent Assay (IQELISA™) is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of lower sample volumes while also providing more sensitivity. The RayBio® Custom Human Activin R2A IQELISA™ Kit is a modified ELISA assay with high sensitivity qPCR readout for the quantitative measurement of Human Activin R2A in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human Activin R2A coated on a 96-well PCR plate. Standards and samples are pipetted into the wells and Activin R2A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and a detection affinity molecule is added to the plates. After washing away unbound detection affinity molecule, primers and a PCR master mix are added to the wells and data is collected using qPCR. Ct values obtained from the qPCR are then used to calculate the amount of antigen contained in each sample, where lower Ct values indicate a higher concentration of antigen.
Application Notes
Kit Components
**The PCR plate used is a 0.2 ml, non-skirted 96-well plate (ThermoFisher, cat. no.: AB0600). Please ensure compatibility with your PCR machine prior to purchase. For additional information contact technical support (cs@medikonia.com).
Other Materials Required
Protocol Outline
Catalog #: IQH-ACVR2A-1 -- 1 Plate Kit
Catalog #: IQH-ACVR2A-2 -- 2 Plate Kit
Catalog #: IQH-ACVR2A-5 -- 5 Plate Kit
Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.