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Element AVITI24™ System
The AVITI24 System with Teton™ CytoProfiling simultaneously measures a diverse array of molecular features in up to 1 million cells per flow cell for deep multiomic profiling of adherent cells or cell suspensions. From a single sample, you can analyze RNA, proteins, and cell morphology in 24 hours. On the same system, the AVITI24 delivers high-quality next-generation sequencing (NGS) data with uncompromising performance, flexibility, and affordability, and access to a range of assays from whole-genome sequencing to targeted panels.
Highlights
- Analyze RNA, protein, and morphology in a single sample
- Profile up to 2 M cells in 24 hours on dual flow cells
- Capture transcripts and proteins with sensitivity and specificity
- Prepare cell samples in 45 minutes hands-on time
Avidite base chemistry (ABC), the AVITI breakthrough optical design, image processing algorithms, and flexible surface chemistries, enable high-plexity multiomic analysis directly in intact cells fixed and permeabilized onto the Teton flow cell surface. Teton chemistries for RNA, protein, and cell paint analysis use probe or antibody-mediated detection schemes coupled with ABC sequencing to measure RNA and protein expression at subcellular resolution in individual cells. Proprietary cell paint profiling provides visualization of the cell membrane, nucleus, and mitochondria, as well as other cell organelles.
Cell segmentation is performed using cell membrane and nuclear markers coupled with machine learning models validated on diverse cell types, ensuring accurate transcript and protein assignment to the correct cell.
The AVITI24 can detect thousands of transcripts per cell and billions of high-quality transcripts in situ on one Teton flow cell for sensitive detection of genes across a range of expression levels, fueling high-resolution cell typing and pathway analysis
The AVITI24 technology is designed with throughput in mind to enable more cells, more samples, and more experiments to empower researchers to drive transformative discoveries without constraints.
With an imaging area of 10 cm² and dual flow cell architecture, the AVITI24 is capable of profiling up to 2 million cells per run, scaling the number of samples, conditions, or perturbations possible in a single 24-hour run.
Cell paint images on AVITI24 using ABC sequencing readout. The total off-instrument workflow requires 45 minutes of hands-on time to wash, fix, and permeabilize cells and assemble the flow cell. Teton introduces the unique ability to automatically perform probe hybridization or antibody binding, amplification, and sequencing directly onboard the instrument.
Cell paint, RNA, and protein detection is enabled by direct sequencing of AVITI barcodes, which obviates the need for time-consuming decoding steps.
The AVITI24 software suite also includes real-time, onboard cell segmentation, cell assignment, and morphology reporting to accelerate downstream data analysis. Together, these innovations reduce the total time from sample to analysis to under 24 hours.
Teton flow cells are available as either ready-to-use PLL-coated f low cells or uncoated customizable flow cells to support a broad range of cell types and culture conditions. The AVITI24 launches with two fixed Teton 350-plex RNA and 50-plex protein panels for humans focused on deep profiling of the MAPK signaling pathway for cell cycle and apoptosis regulation. The expanding AVITI24 roadmap includes additional fixed and custom panels with expanded plexity, as well as direct in situ sequencing for targeted or untargeted transcriptome analysis to accelerate discovery power.
| Read Count | High output: 1 B reads per flow cell Medium output: 500 M reads per flow cell Low output: 250 M reads per flow cell |
| Accuracy | > 90% Q30 with 2 x 150 and 2 x 75 cycles > 85% Q30 with 2 x 300 cycles > 70% Q50 with Cloudbreak UltraQ™ kits |
| Inputs | Direct loading of linear libraries with Cloudbreak Freestyle™ kits |
| Run Time | ≤ 24 hours 2 x 75 cycles ≤ 38 hours 2 x 150 cycles ≤ 60 hours 2 x 300 cycles |
| - Performance metrics are based on sequencing Element libraries. Actual results might differ due to library type and preparation methods. - Individually addressable lanes and custom recipes can extend run times. - Based on Elevate™ libraries and specific run parameters |
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The following specifications are preliminary. Final specifications are subject to change.
| Analytes | RNA, protein, morphology 100 bp in situ RNA sequencing in 2025 |
| Plex | RNA: 350 targets Protein: 50 targets Morphology: 6 markers |
| Content | MAP Kinase Cell Cycle and Apoptosis Immunology, neuroscience, and custom panels in 2025 |
| Imaging | < 250 nm subcellular spatial resolution with multi-feature cell segmentation |
| Inputs | Adherent cells Cell suspensions in 2025 |
| Transcripts | 3000 mean transcripts detected per cell |
| Throughput | Up to 1 M cells with 10 cm² area per flow cell Two flow cells per run |
| Format | 12 wells (0.5 cm²/well) 1 well (10 cm²/well) |
| Run Time | 24 hours |
| Sample Prep | 45 minutes |
| Instrument Configuration | Dual flow cells AVITI Operating Software Ubuntu Core 20.04 LTS operating system |
| Operating Environment | Temperature: 18°C to 26°C Elevation: < 2000 m Sound level: ≤ 62 db at 3.3 ft |
| Instrument Dimensions | (H x W x D) 29.5 in x 37.6 in x 28.5 in Weight: 155.1 kg/342 lb |
| Power Requirements | 100–240 VAC at 50/60 Hz 15 A, 550 W (average) |
Catalog #: 880-00004
Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.
General Enquiry: cs@medikonia.com | Support: support@medikonia.com
+852 2866 8995
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