Yokogawa Single Cellome™ Unit SU10
Brochures Technical Support Single Cellome Unit SU10 enables the delivery of substances, such as genome editing tools, directly to the cytoplasm or nucleus of targeted single cells. For use with an inverted optical microscope. The microscope is not included with the SU10.
- Single-cell targeting with direct delivery into nucleus or cytoplasm.
- Minimal damage to cells.
- Automated, high speed, and high success rate.
FITC-labelled dextran solution (molecular weight 70,000) was delivered into HeLa cells.
The tip of the nanopipette under an electron microscope.
A1. The SU10 lowers the damage to a cell with the nanopipette because its tip size is less than 1/10 of a tip used for microinjection.
Automatic detection of cell surface enables a high success rate of insertion to the intended depth of a cell.
The delivery operation uses an electrical method rather than pneumatic or hydraulic pressure.
A2. The SU10 can deliver materials into the selected cells.
The SU10 enables direct delivery of reagents into the cytoplasm or nucleus.
A3. In addition to the above-mentioned “difference from traditional transfection methods”, due to automated cell surface detection, the suspension of cells is not required during injection.
A4. It is estimated to be tens of femtoliter (fL) per second (1fL=1x10-15L).
The volume can be changed by software settings.
* The delivery volume may vary depending on the solute and vehicle.
A5. Yes, but one nanopipette can deliver to 50 cells or more*.
* Experiment using HeLa cells by Yokogawa.
Actuator Module | Coarse movement (Motor actuator) | Stroke: Approx. 50mm/axis (setting resolution XYZ axis: 0.625μm) |
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Fine movement (Piezo actuator) | Stroke:100μm/axis (setting resolution XYZ axis: 10nm, at penetration and extraction: 1nm) |
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Measurement Module | Voltage generation range | -10V to +10V (setting resolution: 10mV) |
Current measurement range | -900nA to +900nA (setting current range:±9V) | |
Power supply | Power consumption (Main controller + Piezo controller) | 100VA or lower |
Supply voltage (Main controller) | 100 to 200V / 220 to 240VAC (Switching not required) | |
Supply voltage (Piezo controller) | 100 to 120V / 220 to 240VAC (model must be specified when placing an order) | |
Power supply frequency (Main controller + Piezo controller) | 50/60Hz | |
External dimensions and weight | Main controller | 260(W) x 99(H) x 280(D) mm, Approx. 2.8kg |
Piezo controller | 236(W) x 88(H) x 273(D) mm, Approx. 4.6kg | |
Actuator module | 270*(W) x 219(H) x 245*(D) mm, Approx. 2.2kg * In case the X and Y axes move in the direction of the maximum size |
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Measurement module | 85(W) x 30(H) x 43(D) mm, Approx. 0.1kg | |
Joystick | 100(W) x 145(H) x 144(D) mm, Approx. 0.3kg | |
Safety guard | 130(W) x 230(H) x 287(D) mm, Approx. 0.7kg | |
Tip outer diameter of nanopipette (in case of SU10ACC-NP01) | Under 100nm (reference value) | |
Operation Environment | 15 to 35℃, 20 to 70%RH No condensation, altitude up to 2000m | |
Microscope compatibility | For use with an inverted optical microscope. * Microscope is not included with the SU10. Please contact Yokogawa to possibly install the SU10 on a different inverted microscope. Installation examples; Olympus IX71, Olympus IX83, Nikon Ti2 |
For more information or ordering the products, please email us at cs@medikonia.com.