Sampling features

• Automated operation.
• Precise pipetting control of location works.
• Sampling with location and morphology information.
• High resolution images and image analysis using confocal microscopy.
• Incubator function for maintaining cell activity.

Use Cases

1. Intracellular components can be sampled at a single-cell level. This includes intracellular components that are difficult to sample by biochemical methods, such as organelles without lipid membranes.
2. By sampling while maintaining the positional information, it is possible to sample and analyze the normal cells adjacent to the cancer cells and the normal cells located away from the cancer cells.
3. Sampling is possible while maintaining morphological information. Allowing cells with different morphological changes can be sampled and compared.
4. Different parts of a neuron, such as a cell body or axon, can be targeted for sampling.
5. Single-cell cloning is possible from specific cells or cells with specific behavior under microscopic observation, such as transfected cells or virus-resistant cells. By combining various image analysis techniques, accurate and efficient cloning is possible.
6. Multiple samples can be collected in the same well for analysis that requires a sample volume.

Details

Subcellular sampling

Unlike existing cell isolation devices, the SS2000 can not only isolate a whole-cell but also sample only the target site inside the cell. It is possible to sample cytoplasm and regions containing target organelles selectively.

After staining of HeLa cell nuclei (blue), cytoplasm (green), and mitochondria (red), a mitochondria-rich region (arrow) of the cytoplasm was sampled.

Maintaining positional and morphological information of cells

Since only the target cells can be sampled without detaching the cells in culture, it is possible to sample while maintaining positional and morphological information.

Normal MDCK cells and green fluorescent-labeled abnormal MDCK cells were co-cultured at a 50:1 ratio. A normal cell adjacent to the abnormal cell exhibiting fluorescent signals (arrows) was sampled.

High usability samples

Samples can be collected on PCR plates and microplates and collect multiple samples in the same well. Samples can also be taken while being held in the glass tip without being ejected. The collection site has a cooling function to suppress sample degradation and an incubator function to maintain the culture environment. These samples can be used for genetic analysis, mass spectrometry, and single-cell cloning.

Live Cell Imaging with Confocal Microscopy

The SS2000 utilizes live-cell imaging products developed by Yokogawa. High-speed, high-resolution 3D imaging is possible using our unique confocal microscope technology. Samples can be taken from targeted cells under a confocal microscope in an incubator environment. Time-lapse photography is also possible, allowing dynamic changes in the target cell to be captured. Since it is possible to record moving images during sampling and images before and after sampling, it is possible to compare the results of analysis of collected samples with cell imaging data.

Target cells and sampling positions can be automatically selected by image analysis. (Targets can be automatically selected as shape of cells, size of nuclei, density of organelles etc.)

Product Specifications

*1 A sample holder is required, and with it, up to 3 samples can be installed.
*2 A sample holder is required, and with it, up to 4 samples can be installed.

Downloads

Brochures

•  Single Cellome System SS2000 Catalog   (13.5 MB)

For more information or ordering the products, please email us at cs@medikonia.com.