Product Overview

Stellate cells are a resident cell type of the liver primarily functioning to store retinoids. In response to liver damage, stellate cells rapidly lose the stored retinoids and differentiate into a proliferating fibroblast-like cell that begins depositing collagen matrix. This activity causes buildup of collagen in the liver eventually leading to cirrhosis.

Stellate cells can be isolated from liver tissue, enriched, and placed into cell culture. When placed into culture, stellate cells begin to immediately differentiate into the activated state.

Human Cryopreserved Stellate Cells, 100K cells/vial,  are stellate cells enriched from human liver isolations and grown to >80% confluency and then cryopreserved.

Human Stellate Growth Medium (Catalog MCST250) is available to support optimal culture health and growth characteristics of stellate cells.

Characterization performed:

• Post-thaw plating to observe characteristic stellate morphology. 

• Flow cytometry using antibodies for Vinculin, Vimentin, and α-SMA

Benefits

• Low Passage

• High activation potential

Applications

• ADMETox

• Liver Disease

• NASH

Content

Cryo Vial ≥100,000 cells/ vial

Maintenance Media

Lonza Human Stellate Cell Growth Media

Catalog #: HUCLS

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Technical Instructions for culturing human stellate cells

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved