Product Overview

Bone marrow CD34⁺ cells contain hematopoietic stem and progenitor cells and are known to differentiate into all the various blood cell types.

Purity and Viability You Can Count On

Lonza bone marrow CD34⁺ cells are isolated from bone marrow mononuclear cells (MNC) by positive immunomagnetic separation and are guaranteed to have a purity of ≥90%. They are guaranteed to contain either ≥100,000, ≥300,000, ≥500,000, ≥1 million, or ≥2 million viable cells per ampoule depending on the product ordered. HIV-1, hepatitis C, and hepatitis B are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Fresh bone marrow CD34⁺ cells are offered in various packaging sizes and require a minimum order quantity of two vials per donor. Please contact Customer Service for information on sizes and pricing for fresh cells.

Supported by an Optimized Culture System

HPGM™ Hematopoietic Growth Medium is recommended for optimal performance in serum-free culturing (additional cytokines may be necessary depending on application) Alternative media containing serum may also be acceptable. Please contact Scientific Support for serum containing media recommendations.

Choices in Cell Type and Tissue Source

Complex research often calls for flexibility. That's why we offer isolated and purified bone marrow CD34⁺ cells as well as unprocessed bone marrow in case you need to isolate your own cells. Bone marrow CD34⁺ cells are ideal for studies requiring a large number of cells from a single donor or studies investigating differences in cells of donors of various ages, genders, or ethnicities. If however, your studies demand more naïve cells, cord blood CD34⁺ cells may be the better choice.

Lonza guarantees the performance of Clonetics™/Poietics™ cells only if appropriate Clonetics™/Poietics™ media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.

Benefits

• Ready to use

• High purity with > 90% CD34+ cells 

• Wide donor variety

• Large lot sizes (if large lot sizes are not required and more naive HSCs are desirable you may be interested in cord blood derived CD34+/- cells)

Content

1 x Cryopreserved ampule Human Bone Marrow CD34+ containing ≥100,000/ 300,000/ 500,000/ 1 million/ 2 million cells

Recommended Media

Lonza X-VIVO™ 10 with L-glutamine, gentamicin and phenol red

Lonza X-VIVO™ 10 Serum-free Hematopoietic Cell Medium

Lonza X-VIVO™ 15 with L-glutamine, gentamicin, and phenol red

Lonza X-VIVO™ 15 Serum-free Hematopoietic Cell Medium, 500mL

Lonza X-VIVO™ 15 Serum-free Hematopoietic Cell Medium,1L

Lonza X-VIVO™ 15 with with L-glutamine, gentamicin, recombinant transferrin, and phenol red

Catalog #: 2M-101 -- ≥100,000 cells

Catalog #: 2M-101A -- ≥300,000 cells

Catalog #: 2M-101B -- ≥500,000 cells

Catalog #: 2M-101C -- ≥1 million cells

Catalog #: 2M-101D -- ≥2 million cells

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Instructions and Technical Sheet- Human CD34+ Cells

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved