Product Overview

Dendritic Cells (DCs) act as the messenger cells of the immune system by processing antigens from pathogens and presenting the antigens to T cells to initiate an immune response. Poietics™ Normal Human Dendritic Cells are tested for a panel of specific surface markers, including CD11c+, CD86+, CD80+. HLADR+, and CD14- to provide detailed characterization of the cells. These cells can survive up to 7 days in culture with the proper cytokines (IL-4 and GM-CSF) to maintain the cells as immature dendritic cells (iDCs). Cells can be further differentiated to mature dendritic cells with the addition of TNF-α and IL-1β. 

Applications: Antigen presentation and uptake Primary Research Pharmacology Drug-induced immunosuppression of cytokine secretion

Cryopreserved Poietics™ Normal Human Dendritic Cells (NHDC) are guaranteed to contain ≥3 million viable cells per ampoule. Expression levels are tested for CD80, CD86, HLA-DR, CD11c, and CD14 and provided for information only. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Recommended Medium: LGM-3™ Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4

Lonza guarantees the performance of Clonetics™/Poietics™ cells only if appropriate Clonetics™/Poietics™ media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. 

Benefits

• All cells test negative for mycoplasma, bacteria, yeast, and fungi

• HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots

• Certificate of Analysis provided for every cell lot

Content

1 x Cryopreserved ampule of NHDC containing ≥2.5 million cells

Recommended Media

Lonza X-VIVO™ 20 with L-glutamine, gentamicin and phenol red

Lonza X-VIVO™ 20 Serum-free Hematopoietic Cell Medium

Catalog #: CC-2701

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Instructions For Proper Use

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved