Product Overview

Lonza is your ideal partner when you are looking to buy PBMC. Our Peripheral Blood Mononuclear Cells (PBMCs) are isolated from peripheral blood by apheresis and density gradient separation. They are guaranteed to contain ≥50 million viable cells per ampoule. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Ability to Screen Different Donors

Sample representation in your experiment matters. We maintain an inventory of different donors in an effort to satisfy your sample representation requirements. You can choose a lot based on donor demographic characteristics, and screen multiple lots to find the lot(s) that work best for you. In addition, we can request repeat donors if you need additional cells from a particular donor.

Rich Source of Immune Cells

Peripheral blood mononuclear cells (PBMC) are a mixed population of single nucleus cells. They can be further purified to isolate individual cell types such as natural killer cells, T cells, and B cells. In addition, PMBCs are a rich source of monocytes, which can be directed to differentiate into either macrophages or dendritic cells through culture with various cytokines. Supported by an Optimized Media System LGM-3™ Lymphocyte Growth Medium was specifically designed for support and maintenance of lymphocytes and is optimized for serum-free culturing (additional cytokines may be necessary depending on application) Alternative media containing serum may also be acceptable.

Please contact Scientific Support for serum containing media recommendations. Lonza guarantees the performance of Clonetics™/Poietics™ cells only if appropriate Clonetics™/Poietics™ media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.

Benefits

• Test negative for mycoplasma, bacteria, yeast, and fungi

• HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots

• Certificate of Analysis (CoA) is provided for each cell lot purchased

Applications

• ELISPOT

• Proliferation assays

• Immunogenicity assays

• Cell therapy process development

• Humanized mice

• Biomarker discovery

Content

1 x Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥10/ 25/ 50/ 100 million cells

Recommended Media

Lonza X-VIVO™ 20 with L-glutamine, gentamicin and phenol red

Lonza X-VIVO™ 20 Serum-free Hematopoietic Cell Medium

Catalog #: 4W-270 -- ≥10 million cells

Catalog #: 4W-270A -- ≥25 million cells

Catalog #: 4W-270C -- ≥100 million cells

Catalog #: CC-2702 -- ≥50 million cells

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Instructions For Proper Use

Reference guidefor human and animal cells from normal to diseased, fresh and cryopreserved