For concentrating and purifying AAV vectors from cell lysate and cell culture media

• - AAV Purification from any input – cell fraction or media fraction

• - High AAV recovery, up to 90%

• - No specialized equipment needed

• - Purification from a variety of AAV serotypes (including AAV6 and AAV9)

• - Yields highly active AAV for in vivo and in vitro experiments

• - Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

AAV Purification Kits

Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV Purification Kits provide fast and simple procedures for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on precipitation onto Norgen Biotek’s proprietary resin. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro and in vivo transduction experiments.

Norgen’s AAV Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant (SN) or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit. Up to 33X sample concentration.

Kit Specifications

Resin Binding Capacity (total per kit):

• At least 5 x 1010 AAV particles as determined by qPCR

AAV Vector Serotype:

• AAV6, AAV9 and others

Input Type:

• Cells, media

Input Volume (AAV supernatant):

• 1 - 33.5 mL SN per prep (500 mL SN in total)

Input Volume (AAV cell pellet):

• 1 mL cell pellet per prep (15 mL in total)

Time to Complete Purifications:

• 2.5 to 4.5 hours with 1 hour hands on time

In vivo transduction:

• Yes

Storage Conditions and Product Stability

HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

Catalog #: 66100

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

AAV Purification Kit - Protocol (15 preps)

Application Notes

Purification of Adeno-associated Virus (AAV) Vectors Using Norgen’s AAV Purification Kit