All-in-one system for the purification of exosomes and the sequential isolation of RNA and free-circulating RNA from plasma/serum samples

• - Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.

• - ​Versatile sample input ranges

• - Isolate all sizes of free-circulating RNA, including microRNA

• - Bind and elute all RNA irrespective of size or GC content, without bias

• - The purified exosomal RNA is free from any circulating RNA-binding proteins

• - No phenol extractions, Proteinase K treatment, nor carrier RNA

• - No time-consuming ultracentrifugation, filtration nor special syringes are required

• - No precipitation reagents, nor overnight incubation required

• - Concentrate isolated exosomal RNA and are free-circulating RNA into a flexible elution volume ranging from 50 µL to 100 µL

• - Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Plasma/Serum Exosome and Free-Circulating RNA Isolation Kits

These kits provide a fast, reliable and convenient method to sequentially isolate and concentrate exosomal RNA as well as Free-Circulating RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA as well as all sizes of the free-circulating protein-bound RNA, including microRNA. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, these kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. Moreover, the free-circulating, protein-bound, RNA is free from any exosomal RNA. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit

For sample volumes ranging from 50 µL to 1 mL.

Kit Specifications

Minimum Plasma Input:

• 50 μL

Maximum Plasma Input:

• 1 mL

Size of RNA Purified:

• All sizes, including miRNA and small RNA (< 200 nt)

Elution Volume:

• 50 - 100 µL

Time to Complete 10 Purifications:

• 35 - 40 minutes

Average Yields*

• Variable depending on specimen

• *Please check page 5 of the product insert for the average yields and the common RNA quantification methods

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Catalog #: 59500

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit - Protocol (50 prep)

Safety Data Sheets

Plasma/Serum Exosome and Free-Circulating RNA Isolation Kit - Safety Data Sheet