All-in-one system for the purification of exosomes and the subsequent isolation of RNA from plasma/serum samples

• - Purification and enrichment of intact plasma/serum exosomes for functional studies
• - Bind and elute all RNA irrespective of size or GC content, without bias
• - Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias
• - Versatile plasma/serum input volume
• - No phenol extractions, Proteinase K treatment, nor carrier RNA required
• - No time-consuming ultracentrifugation, filtration nor special syringes required
• - No precipitation reagents, nor overnight incubation required
• - Compatible with plasma/serum from any species
• - Pure exosomes are purified and are free-from any other RNA-binding proteins
• - Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
• - The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen's NGS services

For research use only and NOT intended for in vitro diagnostics.

Plasma/Serum Exosome Purification and RNA Isolation Kits

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Kits constitute all-in-one systems for the purification of exosomes and the sequential isolation of exosomal RNA and free-circulating RNA from different plasma/serum sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. These kits are designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen’s Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit

For sample volumes ranging from 50 µL to 1 mL. This kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL.

Kit Specifications

Minimum Plasma/Serum Input:

• 50 μL

Maximum Plasma/Serum Input:

• 1 mL

Size of Exosomes Purified:

• 40 nm - 150 nm

Size of RNA Purified:

• All sizes, including miRNA and small RNA (< 200 nt)

Elution Volume:

• 50-100 μL

Time to Complete 10 Purifications:  

• 35 - 40 minutes

Average Yields*:

• Variable depending on specimen
• *Please check page 4 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Catalog #: 58300

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Plasma/Serum Exosome Purification and RNA Isolation Mini Kit - Protocol (50 prep)

Application Notes

Small RNA Sequencing of Plasma Exosomes Collected on Heparin: Quality and Quantity Challenges

Safety Data Sheets

Plasma/Serum Exosome Purification and RNA Isolation Kit - Safety Data Sheet

Product Flyers

Exosome Purification