All-in-one system for the purification of exosomes from different plasma/serum sample volumes

• - Purification and enrichment of intact exosomes from plasma, serum, urine, cell culture media and saliva in less than 30 minutes.

• - Versatile sample input ranging from 50 µL to 10 mL

  • - Plasma/Serum Exosome Purification Mini Kit (50 µL - 1 mL Plasma/Serum)

  • - Plasma/Serum Exosome Purification Midi Kit (1 mL - 4 mL Plasma/Serum)

  • - Plasma/Serum Exosome Purification Maxi Kit (4 mL - 10 mL Plasma/Serum)

• - Exosome purification is based on Norgen’s proprietary resin separating matrix through exosomes’ surface proteins.

• - No precipitation reagents, overnight incubation, protease or coagulant treatments required

• - No time-consuming ultracentrifugation, filtration or special syringes required

• - Purify intact exosomes with a size ranging from 40-200 nm depending on sample input type

• - Purified exosomes are compatible with functional studies.

• - Purified exosomes are free from any protein-bound cell-free circulating RNA

• - Purified exosomes are compatible with NanoSight® or Electron Microscopy for assessing the approximate exosome size range and concentration.

• - Exosomal RNA can be extracted from the purified exosomes using Norgen’s Exosomal RNA Purification technology or any other RNA extraction method.

For research use only and NOT intended for in vitro diagnostics.

Plasma/Serum Exosome Purification Kit

The Plasma/Serum Exosome Purification Kits provide a fast, reliable and convenient method to purify and enrich for pure intact exosomes from different plasma/serum sample volumes ranging from 50 µL to 10 mL. These kits also allow for the purification of intact extracellular vesicles (EVs) from different plasma/serum sample volumes, and these EVs are ready for any downstream application. The purification is based on Norgen’s proprietary resin.

These kits provide a clear advantage over other available methods since they do not require any special instrumentation, ultracentrifugation, precipitation reagents or any protease treatments. More importantly, the purified exosomes will not be contaminated with any other RNA-binding proteins that may contaminate your exosomal RNA, which is essential if studying exosomal RNA gene expression.

Exosmal RNA Analysis

Exosomal RNA can be isolated using Norgen’s Exosomal RNA Isolation Kit from exosomes enriched using Norgen’s Plasma/Serum Exosome Purification Kits for gene expression analysis using RT-qPCR, microarray or NGS and for Biomarker discovery. Exosomal RNA isolation is based on Norgen’s proprietary resin without the need for phenol extractions or carrier RNA.

Kit Specifications

Plasma/Serum Input:

• 50 μL - 1 mL

Plasma/Serum Input: 

• 1 mL - 4 mL

Plasma/Serum Input:

• 4 mL - 10 mL

Size of Exosomes Purified:

• 40 nm - 150 nm

Elution Volume:

• Variable depending on the plasma/serum input volume

Time to Complete 10 Purifications    

• 15 - 30 minutes

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Important Note
This kit is suitable for the purification of exosomes from fresh or frozen serum or plasma prepared from blood collected on either EDTA or Citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications such as RT-PCR.

Catalog #: 57400

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Plasma/Serum Exosome Purification Kits - Protocol (50 prep)

Safety Data Sheets

Plasma/Serum Exosome Purification Kits - Safety Data Sheet

Product Flyers

Exosome Purification