Fast and simple detergent-free isolation of total proteins

• Simple protocol that is free of detergents

• Fast processing time

• Process a variety of inputs

• Compatible with mass spectrometry and NMR spectroscopy

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Detergent-Free Total Protein Isolation Kit

This kit provides a fast and simple procedure for the isolation of total proteins from tissue, bacteria, yeast or mammalian cells without the use of SDS, Triton® X-100 and other detergents. Detergents are extensively used to prepare protein samples; however, these detergents have undesirable effects on downstream analysis. These effects include extraneous peaks in mass spectrometry, artifacts with chromatography and electrophoresis, interference with microinjection into cells and interference with protein immunization. 

The Detergent-Free Total Protein Isolation Kit maintains high protein recovery and yields proteins that are 100% detergent-free.  Purification is based on using Norgen’s proprietary resin together with Lysis Solution, followed by protein filtration using a filter column (provided). The purified proteins can be used in a number of downstream applications including mass spectrometry, SDS-PAGE, isoelectric focusing, NMR spectroscopy and more.

Each Lysis Tube is able to process up to 50 mg of tissue, 10¹⁰ bacterial cells, 10⁹ yeast cells or 10⁷ mammalian cells. Preparation time for 12 samples is less than 10 minutes. 

Kit Specifications

Maximum Amount Of Starting Material:

• - Tissues: 50 mg

• - Animals Cells: 1 x 10⁷ cells

• - Bacteria: 1 x 10¹⁰ cells

• - Yeast: 1 x 10⁹ cells

Time to Process 12 Samples : 

Less than 10 minutes

Storage Conditions and Product Stability
The Lysis Solution should be kept tightly sealed and stored at room temperature. Once opened, the solution should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

Catalog #: 30300

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Detergent-Free Total Protein Isolation Kit - Protocol (25 prep)

Safety Data Sheets

Detergent-Free Total Protein Isolation Kit - Safety Data Sheet

Product Flyers

Detergent-Free Total Protein Isolation Kit