For the rapid depletion of abundant proteins from serum samples

• Rapid and efficient removal of abundant proteins from serum and plasma samples

• Process 10 samples in 30 minutes

• Allows for visualization of low abundance proteins

• Convenient and affordable spin column protocol

• Generic protocol allows for depletion from human and other animal samples

• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

ProteoSpin™ Abundant Serum Protein Depletion Kit

This kit provides a fast and simple procedure for the effective depletion of major serum proteins including albumin, α-antitrypsin, transferrin and haptoglobin from serum and plasma samples.  Such abundant proteins usually obscure less abundant proteins in gel electrophresis making them difficult to visualize and recover.  The kit reduces sample complexity to allow for convenient investigation of less abundant proteins.  In other applications such as mass spectroscopy, fractionating abundant proteins improves the resolution of less abundant proteins.  The kit is 'generic' in that it is not antibody-based and can thus be used to deplete abundant proteins from any starting material - human or various other animals - making it more versatile, flexible and affordable than other kits.   Resulting proteins are ready for a range of applications including 2D gel electrophoresis, SDS-PAGE, Mass Spectrometry, Protein microarrays, DIGE (Difference Gel Electrophoresis) and more.

Kit Specifications

Maximum Column Capacity: 500 μg

Minimum Column Capacity: 200 μg

Minimum Elution Volume: 30 μL

Time to Process 10 Samples: 30 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

Catalog #: 17300

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

ProteoSpin™ Abundant Serum Protein Depletion Kit - Protocol (25 prep)

Safety Data Sheets

ProteoSpin™ Abundant Serum Protein Depletion Kit - Safety Data Sheet

Product Flyers

Detergent-Free Total Protein Isolation Kit