For rapid and efficient endotoxin removal from proteins and peptides

• Columns bind proteins of interest while endotoxins flow through

• Reduce endotoxin levels to less than 0.01 EU/µg of protein

• Proteins are desalted

• Greater than 95% protein recovery

• Concentrate protein samples and remove detergents at the same time

• Effectively remove a wide range of detergents including SDS, Triton® X-100, CHAPS, NP-40, and Tween 20

• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Kits are designed for the rapid removal of endotoxins from previously purified proteins or peptides, with protein recoveries of > 95% being achieved. Endotoxins, also known as lipopolysaccharides, are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins liberated by Gram-negative bacteria are frequent contaminations of protein solutions derived from bioprocesses. Due to the high toxicity of endotoxins in vivo and in vitro, their removal from protein preparations is often necessary prior to the use of the protein in downstream applications. These kits efficiently reduce endotoxin levels to ≤ 0.01 EU/mg of protein, using spin column chromatography based on Norgen’s proprietary resin as the separation matrix. These kits are highly efficient in removing many different salts commonly used in the laboratory including, but not limited to, MgCl₂, NaCl, KCl, CaCl₂, LiCl and CsCl. The purified protein samples can be used in a number of downstream applications including sequencing, cloning, and in vitro and in vivo introduction into cells and organisms for various purposes. The simultaneous removal of salts while concentrating a dilute protein solution makes the kit a convenient method for preparing proteins before running many downstream applications such as SDS-PAGE, isoelectric focusing, X-ray crystallography, NMR spectroscopy, mass spectroscopy and other applications.

Mini Kit

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit is designed for the rapid removal of endotoxins from up to 200 μg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.

Maxi Kit

The ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit is designed for the rapid removal of endotoxins from up to 4 mg of previously purified proteins or peptides, with protein recoveries of > 95% being achieved.

Kit Specifications -- Mini Kit (25 preps) (Catalog #: 22800)

Maximum Protein Input: 200 μg

Maximum Column Volume Input: 600 μL

Molecular Weight of Recovered Proteins: No Molecular Weight cut-off

Final Endotoxin Levels: ≤ 0.01 EU/μg protein

Protein Recovery: 90-95%

% Detergent Removal: 90-95%

Detergents that can be Removed: Including Triton® X-100, CHAPS, NP-40 and Tween 20

Minimum Elution Volume: 50 μL

Time to Complete 10 Purifications: 20 minutes

Kit Specifications -- Maxi Kit (4 preps) (Catalog #: 22200)

Maximum Protein Input: 4 mg

Minimum Protein Inpput: 0.25 mg

Maximum Column Volume Input: 18 mL

Molecular Weight of Recovered Proteins: No Molecular Weight cut-off

Final Endotoxin Levels: ≤ 0.01 EU/μg protein

Protein Recovery: 90-95%

% Detergent Removal: 90-95%

Detergents that can be Removed: Including Triton® X-100, CHAPS, NP-40 and Tween 20

Elution Volume: 2-4 mL

Time to Complete 10 Purifications: 30-40 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Catalog #: 22800 -- Mini Kit (25 preps)

Catalog #: 22200 -- Maxi Kit (4 preps)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit - Protocol (25 preps)

ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit - Protocol (4 prep)

Application Notes

Rapid Concentration of Dilute Protein Solutions Using the ProteoSpin™ CBED Kit

Rapid Desalting of Protein Solutions Using the ProteoSpin™ CBED Kit

Efficient Removal of Both Free and Tightly-Bound SDS from Protein Solutions

Efficient Removal of Triton X-100 Prior to MALDI-TOF and Digestion

Safety Data Sheets

ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Mini Kit - Safety Data Sheet

ProteoSpin™ Total Protein Concentration, Detergent Clean-Up and Endotoxin Removal Maxi Kit - Safety Data Sheet

Product Flyers

Detergent-Free Total Protein Isolation Kit

Endotoxin Removal Kits