For the rapid isolation of inclusion body proteins

• All-in-one solution for inclusion body protein isolation and purification

• Fast and convenient spin column protocol

• Complete kit with Cell Lysis Reagent, Inclusion Body Solubilization Reagent, buffers and spin columns to purify proteins

• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

ProteoSpin™ Inclusion Body Protein Isolation Kits

These kits provide everything required to isolate and purify inclusion body proteins from induced bacterial cultures. First a proprietary Cell Lysis Reagent is used to selectively lyse the cells and release inclusion bodies in their solid form. Next, inclusion bodies are dissolved and their contents released using the provided IB Solubilization Reagent. Inclusion body proteins are then further purified using spin columns for rapid and convenient buffer exchange and desalting. This kit provides a convenient way to screen recombinants prior to scaling up.

ProteoSpin™ Inclusion Body Protein Isolation Micro Kit

The process is efficient and streamlined and can process up to 12 samples in only 60 minutes. Each spin column is able to recover up to 50 µg of acidic or basic proteins. Purified recombinant proteins are then ready for SDS-PAGE, 2D gels, Western blots, Mass Spectrometry analysis, and other applications.

ProteoSpin™ Inclusion Body Protein Isolation Maxi Kit

The procedure is efficient and streamlined and can process up to 4 samples in approximately 2 hours. Each spin column is able to recover up to 12 mg of acidic or basic proteins from 100 mL of induced bacterial culture. Purified recombinant proteins are then ready for SDS-PAGE, 2D gels, Western blots, Mass Spectrometry analysis, and other applications.

About Inclusion Bodies

Bacteria are widely used for the expression of different proteins. However, 70-80% of the proteins expressed in bacteria by recombinant techniques are typically contained in insoluble inclusion bodies (i.e., protein aggregates). The protein of interest found in these subcellular structures is often inactive, due to incorrect folding. The production rate of recombinant proteins stored in inclusion bodies is invariably higher than those synthesized as soluble proteins. The reason behind this is thought to be the resistance of insoluble proteins to proteolysis by cellular enzymes. In addition, separation of insoluble recombinant proteins in inclusion bodies is considerably easier than that of soluble proteins. These factors have been the major influences favoring scale-up of high-value proteins using bacterial fermentation for example. Procedures for the purification of the expressed proteins from inclusion bodies are often labour-intensive, time-consuming and not cost-effective. This kit provides the essential reagents for cell disruption, inclusion body solubilization and purification using spin column chromatography - all optimized to work together thereby simplifying the process and saving a tremendous amount of time and cost.

Kit Specifications -- Micro Kit (25 preps) (Catalog #: 10300)

Maximum Culture Volume: 1.5 mL

Yield from 1.5 mL: Up to 50 μg

Minimum Elution Volume: 30 μL

Time to Process 12 Samples: 60 minutes

Kit Specifications -- Maxi Kit (4 preps) (Catalog #: 17700)

Maximum Culture Volume: 100 mL

Yield from 100 mL of Culture: Up to 12 mg

Minimum Elution Volume: 4 μL

Time to Process 1Samples: 1 hour

Storage Conditions
The Cell Lysis Reagent and IB Solubilization Reagent should be stored at 4°C upon receipt of this kit. This kit is stable for 2 years after the date of shipment. Once opened, the solutions should be stored at 4°C when not in use except for Binding Buffer C and Binding Buffer N. Some precipitation will occur with 4°C storage. This precipitation should be dissolved with slight heating to room temperature before using.

Catalog #: 10300 -- Micro Kit (25 preps) 

Catalog #: 17700 -- Maxi Kit (4 preps) 

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Inclusion Body Isolation Micro Kit - Protocol (25 prep)

Inclusion Body Isolation Maxi Kit - Protocol (4 prep)

Application Notes

Screening For Bacterial Clones Expressing Inclusion Body Proteins

A Time Course Induction Study of Recombinant Protein Expression In E Coli

Safety Data Sheets

Inclusion Body Isolation Micro Kit - Safety Data Sheet

Inclusion Body Isolation Maxi Kit - Safety Data Sheet

Product Flyers

Detergent-Free Total Protein Isolation Kit