For the rapid and efficient extraction and purification of RNA (including microRNA) from FFPE samples

• Extract total RNA (including microRNA) from FFPE samples

• No phenol extraction step

• Includes DNase for optional on-column DNA removal

• Isolated RNA is of the highest quality and integrity

• Isolate a diversity of RNA species

• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing.

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

FFPE RNA Purification Kits

Norgen’s FFPE RNA Purification Kits provide a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. Norgen’s FFPE RNA Purification Kits provide conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. These kits are able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. The RNA is preferentially purified from other cellular components without the use of phenol or chloroform.

Maximum loading volume of 650 μL per column, and a maximum binding capacity of 50 μg per column.

Kit Specifications

Maximum Column Binding Capacity: Up to 50 µg RNA

Maximum Loading Volume Per Spin Column: 650 µL

Size of RNA Purified: All sizes, including small RNA (< 200 nt)

Time to Complete 10 Purifications: 1-4 hours*

Maximum Amount of Starting Material:

• 5 slices of < 20 µm thick paraffin slices

• 25 mg of unsectioned block

Average Yield:

• Variable due to age of paraffin blocks

• ~2-3 µg of Total RNA per 1 mg of fresh FFPE hamster kidney

* Time required for purification varies by length of Proteinase K incubation and formalin crosslink-reversal

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The DNAse I and Proteinase K should be stored at -20°C upon arrival. This kit is stable for 1 year from the date of shipment.

Catalog #: 25300

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

FFPE RNA Purification Kit (Spin Column) - Protocol (50 prep)

Application Notes

Rapid Method for the Purification of Total RNA from Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Samples

Revised Guidelines for RNA Quality Assessment for Diverse Biological Sample Input

Safety Data Sheets

FFPE RNA Purification Kits - Safety Data Sheet