For isolating the highest quality and quantity of all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA from various amounts of plasma/serum with minimal DNA contamination

• Versatile plasma/serum input ranging from 1 mL to 6 mL

• No phenol extractions

• No carrier RNA

• Bind and elute all RNA irrespective of size or GC content, without bias

• Concentrate circulating RNA and exosomal RNA into a flexible elution volume ranging from 25 µL to 50 µL

• Purify superior-quality and superior quantity RNA in 45 minutes

• Fully automated purification procedure on Hamilton MicroLab Nimbus

• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. 

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Plasma/Serum cfc-RNA Advanced Purification Kit

Norgen’s Plasma/Serum cfc-RNA Advanced Purification Kit provides a fast, reliable and simple procedure for isolating the highest quality and quantity of all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA from various amounts of plasma/serum ranging from 1 mL up to 6 mL with minimal DNA contamination. Purification is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-RNA from either fresh, preserved or frozen plasma/serum samples. Moreover, this kit allows the user to elute the purified cfc-RNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-RNA is eluted in an Elution Solution that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

Kit Specifications

Minimum Plasma/Serum Input: 1 mL

Maximum Plasma/Serum Input: 6mL

Size of cfc-RNA Purified: All sizes, including miRNA and small RNA (< 200 nt)

Elution Volume: 25-50 µL

Time to Complete 10 Purifications: 45 minutes

Average Yields*: Variable depending on specimen

*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common RNA Quantification Methods.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Catalog #: 68200

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Plasma/Serum cfc-RNA Advanced Purification Kit - Protocol (50 Preps)