Purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA

• Isolate all sizes of circulating DNA from fresh, preserved or frozen urine samples

• Isolate viral DNA

• Versatile urine input volumes

• Concentrate circulating DNA into flexible elution volumes

• Isolate inhibitor-free cell-free circulating DNA

• Compatible with Norgen’s Urine Preservative and other commercially available urine preservatives

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Urine Cell-Free Circulating DNA Purification Kits

These kits provide a fast, reliable and convenient method to purify and concentrate high quality, high purity and inhibitor-free cell-free circulating DNA as well as viral DNA from fresh, preserved or frozen urine samples. All components for the purification are provided in one convenient and fast kit for the easy processing of small input volumes of bodily fluids. The purified urine DNA is fully compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, Microarrays and NGS.

Background

Recent evidence indicates that cell-free circulating DNA (cf-DNA) contains valuable information for the discovery of biomarkers that can help with early detection of certain cancer types and for monitoring the disease status. The advantage for using urine as a source for cancer biomarkers is that it can be acquired in large quantities without using invasive procedures. In addition, repeated sampling from the same individual is applicable, which facilitates longitudinal studies. There are many advantages favouring the use of urinary nucleic acids for cancer biomarker discovery over blood, tissue samples or other bodily fluids, including: (1) urine is non-infectious for HIV and less infectious for many other pathogens; (2) the profile of urinary nucleic acid is similar to that in plasma or serum; (3) nucleic acid purification from urine is technically much easier because of its low protein concentration (1000-fold lower than blood). Urine cell-free circulating DNA (cf-DNA) has been utilized for the early diagnosis, prognosis and monitoring of therapy for several cancer types and autoimmune diseases, as well as for investigating fetal DNA that is normally present in the maternal blood. This cf-DNA is usually present as short fragments, generally between 150 and 250 bp or its duplicates, and is derived either from the apoptotic process, necrotic process or from the fetus.

Urine Cell-Free Circulating DNA Purification Mini Kit

For sample volumes ranging from 250 µL to 2 mL.

Urine Cell-Free Circulating DNA Purification Midi Kit

For sample volumes ranging from 2 mL to 10 mL.

Urine Cell-Free Circulating DNA Purification Maxi Kit

For sample volumes ranging from 10 mL to 30 mL.

Kit Specifications -- Mini Kit (50 preps) (Catalog #: 56600)

Minimum Urine Input: 250 μL

Maximum Urine Input: 2 mL

Size of DNA Purified: All sizes of DNA ≥ 50 bp

Elution Volume: 50-100 μL

Time to Complete 10 Purifications: 15-20 minutes

Average Yields: Variable depending on specimen

*Please check page 6 of the product insert for average yields and the common DNA quantification methods.

Kit Specifications -- Midi Kit (20 preps) (Catalog #: 56700)

Minimum Urine Input: 2 mL

Maximum Urine Input: 10 mL

Size of DNA Purified: All sizes of DNA ≥ 50 bp

Elution Volume: 50-100 μL

Time to Complete 10 Purifications: 40-45 minutes

Average Yields: Variable depending on specimen

*Please check page 6 of the product insert for average yields and the common DNA quantification methods.

Kit Specifications -- Maxi Kit (10 preps) (Catalog #: 56800)

Minimum Urine Input: 10 mL

Maximum Urine Input: 30 mL

Size of DNA Purified: All sizes of DNA ≥ 50 bp

Elution Volume: 50-100 μL

Time to Complete 10 Purifications: 40-45 minutes

Average Yields: Variable depending on specimen

*Please check page 6 of the product insert for average yields and the common DNA quantification methods.

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Catalog #: 56600 -- Mini Kit (50 preps)

Catalog #: 56700 -- Midi Kit (20 preps) 

Catalog #: 56800 -- Maxi Kit (10 preps)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Urine Cell-Free Circulating DNA Purification Kits - Protocol (50 Preps)

Urine Cell-Free Circulating DNA Purification Kits - Protocol (20 Preps)

Urine Cell-Free Circulating DNA Purification Kits - Protocol (10 Preps)

Application Notes

Application Note 82 - Determination of the DNA Molecular Weight (MW) from different Norgen Columns and Isolation Methods

Safety Data Sheets

Urine Cell-Free Circulating DNA Purification Kits - Safety Data Sheet