For the rapid removal of endotoxins from previously purified DNA

• Reduce endotoxin levels to 0.1 EU/µg DNA or less

• Available in 3 formats: Mini, Midi, and Maxi to suit your desired input volume

• Remove endotoxins from up to 1 mg of DNA with the Maxi format kit

• Fast and easy processing using a rapid spin-column format

For research use only and NOT intended for in vitro diagnostics.

Endotoxin Removal Kits- For DNA

These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen's spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample.

Endotoxin Removal Kit (Mini)

This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes.

Endotoxin Removal Kit (Midi)

This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.

Endotoxin Removal Kit (Maxi)

This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.

About Endotoxins

Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.

Kit Specifications -- Mini Kit (25 preps) (Catalog #: 22700)

Maximum DNA Input: 25 μg

Final Endotoxin Level: ≤ 0.1 EU/µg DNA

Size of DNA Purified: Up to 13,000 bp

Maximum DNA Volume Input: 100 μL

Average Recovery: > 90%

Time to Complete 10 Purifications: 20 minutes

Kit Specifications -- Midi Kit (10 preps) (Catalog #: 52200)

Maximum DNA Input: 200 µg

Final Endotoxin Level: ≤ 0.1 EU/µg DNA

Size of DNA Purified: Up to 13,000 bp

Maximum DNA Volume Input: 0.75 mL

Average Recovery: > 90% (~80% in First Elution)

Time to Complete 10 Purifications: 30 minutes

Kit Specifications -- Maxi Kit (4 preps) (Catalog #: 21900)

Maximum DNA Input: 1 mg

Final Endotoxin Level: ≤ 0.1 EU/µg DNA

Size of DNA Purified: Up to 13,000 bp

Maximum DNA Volume Input: 3 mL

Average Recovery: > 90%

Time to Complete 4 Purifications: 30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Catalog #: 22700 -- Mini Kit (25 preps)

Catalog #: 52200 -- Midi Kit (10 preps)

Catalog #: 21900 -- Maxi Kit (4 preps)

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Endotoxin Removal Kit (Mini) - Protocol (25 prep)

Endotoxin Removal Kit (Midi) - Protocol (10 prep)

Endotoxin Removal Kit (Maxi) - Protocol (4 preps)

Safety Data Sheets

Endotoxin Removal Kit - Safety Data Sheet

Product Flyers

Endotoxin Removal Kits