For rapid and convenient plasmid DNA preparations

• Isolate high quality, high yield plasmid DNA

• Plasmid DNA is ready for various downstream applications including restriction digestion, bacterial transformation, sequencing and more

• Available in 4 formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), and MaxiPrep

For research use only and NOT intended for in vitro diagnostics.

Plasmid DNA Kits

These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.

Plasmid MaxiPrep Kit

This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.

Kit Specifications -- 4 preps

Column Binding Capacity: 1.5 mg

Average Yield from 100 mL Culture*: 0.4 - 1.0 mg

Final Endotoxin Levels: < 0.1 EU/µg DNA

Time to Complete 4 Purifications: 1.5 hours

Size of Plasmids Purified: Up to 13,000 bp

 * Depends on high-copy DNA and low copy DNA

Kit Specifications -- 20 preps

Column Binding Capacity: 1.5 mg

Average Yield from 100 mL Culture*: 0.4 - 1.0 mg

Final Endotoxin Levels: < 0.1 EU/µg DNA

Time to Complete 4 Purifications: 1.5 hours

Size of Plasmids Purified: Up to 13,000 bp

 * Depends on high-copy DNA and low copy DNA

Catalog #: 46500 -- 4 preps

Catalog #: 46600 -- 20 preps

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Plasmid DNA MaxiPrep Kit - Protocol (4 prep)

Plasmid DNA MaxiPrep Kit - Protocol (20 prep)

Application Notes

Plasmid DNA Purification Using Norgen Preparative Chromatography Columns

Comparison of Stool DNA Isolation Methods for Bacterial and Mammalian DNA Detection

Safety Data Sheets

Plasmid DNA MaxiPrep Kit - Safety Data Sheet