For the rapid and simple purification of bacterial and host DNA from stool and fecal samples

• Simultaneous isolation of both host DNA and microbial DNA  (universal protocol)

• Fully compatible with Norgen's Stool Nucleic Acid Collection and Transport Tubes

• High quality DNA for sensitive downstream applications including PCR, qPCR, Sequencing and microarray

• 96-Well and Magnetic Bead System formats also available

For research use only and NOT intended for in vitro diagnostics.

Stool DNA Isolation Kits

These kits provide a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. Purifies the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA can be used with a number of downstream applications.

Stool DNA Isolation Kit (Spin Column)

Universal method to detect microorganism and host cell DNA simultaneously in stool samples. Eliminates PCR inhibitors including all traces of humic acid using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, NGS and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.

Kit Specifications

Maximum Stool Input: 200 mg (fresh/frozen stool) or 400 μL (preserved stool)

Type of Stool Processed: Frozen, fresh or preserved stool

Format: Spin Column

Maximum Column Binding Capacity: 50 μg

Maximum Column Loading Volume: 650 μL

Elution Volume: 50 μL

Time to Complete 10 Purifications: 30 minutes

Applications: PCR, qPCR, Southern Blot Analysis, Sequencing, Microarray Analysis

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Catalog #: 27600

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Stool DNA Isolation Kit (Spin Column) - Protocol (50 prep)

Supplementary Protocols

Supplementary Protocol - Automated Procedure for Stool DNA Isolation Kit (Magnetic Bead System)

Application Notes

Comparative Study of DNA Isolated from Stool Using Norgen’s Stool Nucleic Acid Isolation Kit and Norgen’s Stool DNA Isolation

Detection of Viral, Bacterial and Human Genomic DNA from Preserved Stool Samples

The Isolation of High Quality Stool DNA and its Application to Quantitative Adenovirus Detection using TaqMan® Real-Time PCR

Comparison of Stool DNA Isolation Methods for Bacterial and Mammalian DNA Detection

Determination of the DNA Molecular Weight (MW) from different Norgen Columns and Isolation Methods

Immediate Viral Inactivation Using Norgen’s Stool Nucleic Acid Preservative

Gut Microbiome Diversity: Comparison of Stool DNA Preservation Methods

Comparison of Manual Stool DNA Isolation to Automated Stool DNA Isolation Using Norgen's Magnetic Bead Stool DNA Isolation Kit

Comparative Microbiome Profiles from Fecal Preservation Methods

Safety Data Sheets

Stool DNA Isolation Kit - Safety Data Sheet

Posters

Viability Of Microorganisms in Saliva & Stool Collection and Transportation Preservatives

Product Flyers

Stool DNA Isolation Kit