For rapid and simple purification of all sizes of cell-free circulating DNA (cfc-DNA) and circulating tumour DNA (ct-DNA) from plasma and serum samples

• Versatile plasma and serum input volumes (0.5 mL - 6 mL)

• Concentrate cfc-DNA and ct-DNA into a flexible elution volume ranging from (25 µL - 50 µL)

• Minimal high molecular weight gDNA contamination in the purified cfc-DNA

• Isolate inhibitor-free cell-free circulating DNA

• Purify superior quantity and quality DNA in 45 minutes

• Compatible with fresh, preserved or frozen serum/plasma prepared from blood collected on either Norgen's cf-DNA/cf-RNA Preservative Tubes (Cat. 63950, 63960), Cell-Free DNA BCT ® (Streck), Heparin, EDTA or Citrate

• Fully automated Isolation procedure on Hamilton MicroLab Nimbus

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Plasma/Serum cfc-DNA Advanced Purification Kit

Norgen’s Plasma/Serum cfc-DNA Advanced Purification Kit provides fast, reliable and simple procedures for isolating the highest quality and quantity of cell-free circulating DNA (cfc-DNA) from various amounts of plasma/serum ranging from 1 mL up to 6 mL with minimal genomic DNA (gDNA) contamination. Isolation is based on using Norgen’s proprietary resin separation matrix. The kit is designed to isolate all sizes of cfc-DNA and ct-DNA from either fresh, preserved or frozen plasma/serum samples. Moreover, this kit allows the user to elute the purified cfc-DNA into a flexible elution volume ranging from 25 µL to 50 µL. The purified plasma/serum cfc-DNA is eluted in an Elution Buffer that is compatible with all downstream applications including PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis, microarrays and NGS.

Kit Specifications

Minimum Plasma/Serum Input: 500 µL

Maximum Plasma/Serum Input: 6 mL

Optimal size of purified cfc-DNA: 146 - 180 bp

Elution Volume: 25-50 µL

Time to Complete 10 Purifications: 45 minutes

*Please check page 5 in the product manual for the Plasma/Serum Average Yields and the Common DNA Quantification Methods.

Storage Conditions and Product Stability

All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.

Catalog #: 68000

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Plasma/Serum cfc-DNA Advanced Purification Kit - Protocol (50 Preps)