A convenient and rapid method to isolate total DNA and RNA from fresh, frozen and preserved stool samples

• Simultaneous isolation of both host and microbial DNA and RNA

• Eliminates PCR inhibitors including humic acids

• High quality total RNA and DNA for sensitive downstream applications

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Stool Nucleic Acid Isolation Kit

This kit provides a rapid spin column method to isolate total DNA and RNA from fresh or frozen stool samples, as well as from stool collected using Norgen's Stool Nucleic Acid Collection and Preservation Tubes. The universal protocol conveniently allows for the isolation of total genomic DNA and total RNA from all the various microorganisms and host cells found in the stool sample simultaneously.  The kit removes all traces of humic acids and provides consistent, high yields of RNA and DNA.

The purified DNA and RNA are of the highest quality and are fully compatible with downstream PCR applications, as all humic acid substances and other PCR inhibitors are removed during the isolation.

Kit Specifications

Maximum Stool Input: 200 mg

Type of Stool Processed: Preserved, frozen, and fresh stool

Maximum Column Binding Capacity: 50 μg

Maximum Column Loading Volume: 650 μL

Time to Complete 10 Purifications: 30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Catalog #: 45600

Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Stool Nucleic Acid Isolation Kit - Protocol (50 prep)

Application Notes

The Isolation of High Quality Stool DNA and its Application to Quantitative Adenovirus Detection using TaqMan® Real-Time PCR

Comparison of Stool DNA Isolation Methods for Bacterial and Mammalian DNA Detection

Comparative Microbiome Profiles from Fecal Preservation Methods

Comparison of DNA Isolated from Stool Using Norgen’s Isolation Kits Versus Competitor’s DNA Stool Mini Kit

Immediate Viral Inactivation Using Norgen’s Stool Nucleic Acid Preservative

Posters

Stool Nucleic Acid Isolation Kit Effect of Stool Transportation Conditions On Microbiota Diversity - Poster