A convenient and rapid method to purify total RNA from small amounts of stool samples

• Simultaneous isolation of both host RNA and microbial RNA (universal protocol)

• Isolate full diversity of RNA from large RNA down to small and microRNAs

• Eliminates PCR inhibitors including humic acids

• High quality RNA for sensitive downstream application

• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

For research use only and NOT intended for in vitro diagnostics.

Stool Total RNA Purification Kit

This kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples, manure and samples collected using Norgen's Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The kit removes all traces of humic acids using rapid and simple spin column procedures. Bead tubes are also provided for effective homogenization of stool. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. Both host and microbial RNA is recovered. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis. The procedure can be completed in approximately 30 minutes for 10 samples.

Kit Specifications

Maximum Stool Input:  200 mg (fresh/frozen stool) or 400 μL (preserved stool)

Type of Stool Processed: Preserved, fresh, and frozen stool from humans and animals 

Maximum Column Binding Capacity: 50 μg

Maximum Column Loading Volume: 600 μL

Time to Complete 10 Purifications: 30 minutes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Catalog #:49500
Please email us at cs@medikonia.com for any enquiry. To place an order, please include the catalog number(s) of the product(s) in the email.

Protocols

Stool Total RNA Purification Kit - Protocol (50 prep)

Application Notes

Purification and Detection of Bacterial RNA from Preserved Stool Samples

Comparative Microbiome Profiles from Fecal Preservation Methods

Preservation of RNA in Soil, Plant and Stool Samples Using Norgen's RNA Preserve

Safety Data Sheets

Stool Total RNA Purification Kit - Safety Data Sheet

Posters

Rapid and qPCR ready DNA isolation from environmental and human bodily fluid samples using Magnetic bead system

Product Flyers

Stool DNA Isolation Kit